The Access Array™ Integrated Fluidic Circuit (IFC) facilitates parallel amplification of 48 unique samples, in effect preparing 48 sequencing libraries, in just a few hours. Every reaction combines both an amplicon tagging and a barcoding (identification) step that enables all 48 samples to be multiplexed at the sequencing step. This powerful chemistry simplifies the up-front preparation and maximizes the utility of today's next generation sequencers.
The Fluidigm Access Array System can be used with any PCR-based
sample preparation method and with the reagents and primers of your
choice. The system includes Access Array IFCs, two IFC Controller AXs,
and the Stand-Alone Thermal Cycler. These components deliver sample
throughput and coverage scalable with laboratory
needs.
Target Enrichment
Target
enrichment refers to the ability to select a specific region of
interest prior to sequencing. For example, if you were interested in
examining 20 specific genes from a large cohort of individuals it would
be both wasteful and prohibitively expensive to sample the entire genome
of each individual. Instead, target enrichment technologies allow you
to select regions for amplification from each individual and thus only
sequence the specific area of interest. |
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Sample Barcoding for Multiplexed Sequencing
One
of the largest challenges facing next-generation sequencing operators
today is how to use the massive amounts of throughput enabled by the
new crop of sequencing equipment. While all of the systems in use today allow
massive amounts of data to be generated on a per-sample basis, they lack
a simple and reliable method for running multiple samples per sequencing run.
Barcoding samples during the target enrichment process enables the users to
pool multiple samples per sequencing run, and deconvolute the sample source during the data analysis step based on the barcode.
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Library Preparation Using Amplicon Tagging
Library preparation for next-generation sequencing is by far the most
time and labor consuming part of the entire next-generation sequencing
process. While necessary for whole genome sequencing studies, the
process can be almost entirely eliminated for resequencing projects by
using PCR and amplicon tagging. By incorporating the adaptor sequences
into the primer design the amplicon product is ready to go directly
into clonal amplification since it already contains the necessary
capture sequences. |
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