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Access Array™ System
Key Application:  Targeted Resequencing

The Access Array System is the first high-throughput, target-enrichment system designed to work with all of the major next-generation sequencing instruments. The Access Array System enables the user to enrich multiple unique targets (such as exons) from a large number of samples, all at one time. The system combines the cost and throughput benefits of microfluidics with the proven performance and flexibility of PCR.

  • Target Enrichment
  • Sample Barcoding for Multiplexed Sequencing
  • Sequencing Library Prep Using Amplicon Tagging

Custom designed primer sets amplify (and tag) PCR products for sequencing. Simply provide Fluidigm with your regions of interest for the human genome.

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Access Array™ System

The Access Array™ Integrated Fluidic Circuit (IFC) facilitates parallel amplification of 48 unique samples, in effect preparing 48 sequencing libraries, in just a few hours. Every reaction combines both an amplicon tagging and a barcoding (identification) step that enables all 48 samples to be multiplexed at the sequencing step. This powerful chemistry simplifies the up-front preparation and maximizes the utility of today's next generation sequencers.

The Fluidigm Access Array System can be used with any PCR-based sample preparation method and with the reagents and primers of your choice. The system includes Access Array IFCs, two IFC Controller AXs, and the Stand-Alone Thermal Cycler.  These components deliver sample throughput and coverage scalable with laboratory needs.


Target Enrichment

Target enrichment refers to the ability to select a specific region of interest prior to sequencing. For example, if you were interested in examining 20 specific genes from a large cohort of individuals it would be both wasteful and prohibitively expensive to sample the entire genome of each individual. Instead, target enrichment technologies allow you to select regions for amplification from each individual and thus only sequence the specific area of interest.

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Sample Barcoding for Multiplexed Sequencing

One of the largest challenges facing next-generation sequencing operators today is how to use the massive amounts of throughput enabled by the new crop of sequencing equipment. While all of the systems in use today allow massive amounts of data to be generated on a per-sample basis, they lack a simple and reliable method for running multiple samples per sequencing run. Barcoding samples during the target enrichment process enables the users to pool multiple samples per sequencing run, and deconvolute the sample source during the data analysis step based on the barcode.

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Library Preparation Using Amplicon Tagging

Library preparation for next-generation sequencing is by far the most time and labor consuming part of the entire next-generation sequencing process. While necessary for whole genome sequencing studies, the process can be almost entirely eliminated for resequencing projects by using PCR and amplicon tagging. By incorporating the adaptor sequences into the primer design the amplicon product is ready to go directly into clonal amplification since it already contains the necessary capture sequences.

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IFC Controller AX

The Fluidigm IFC Controller AX is designed to prime and load 48.48 Access Array IFCs exclusively. One chip at a time, the AX sets up 2,304 reactions quickly and easily.

Key advantages include:

  • Automated setup of 48.48 Access Array IFCs
  • Significantly fewer liquid handling steps compared to conventional platforms
  • Self-contained and fully integrated design
  • Touch screen interface

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IFC Controller AX

Fluidigm FC1 Cycler

The Fluidigm FC1 Cycler is specially designed for thermal cycling of Fluidigm Dynamic Array and Digital Array IFCs.

Key advantages include:

  • Highly efficient workflow for all applications
  • Easy scalability to meet throughput requirements
  • Familiar, easy to use interface

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FC1 Cycler


Access Array IFC

The Access Array IFC is the first commercially available microfluidic device which allows the user to recover the PCR product. It automatically assembles 2,304 unique PCR reactions, each reaction a combination of one of the 48 samples and one of the 48 primer inlets. Post PCR the product of 48 reactions per sample is harvested out of the Access Array IFC for downstream sequencing.


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48.48 Access Array IFC

EGFR/MET Panel

The EGFR/MET panel for human DNA is optimized to work with the Access Array System enabling users to quickly and easily resequence the EGFR and Met gene exons. The panel is ideal for any researcher interested in lung cancer research.


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Access Array™ Target-specific Primers

Take full advantage of your Access Array™ System with Fluidigm Access Array Target-specific Primers. Simply provide us with your regions of interest and we will design primer sets to amplify (and tag) PCR products for sequencing. When combined with Fluidigm’s Barcode Library, 48 amplicons per chip will be uniquely barcoded with the necessary sequencing adaptors ready for clonal amplification.

Benefits include:

  • Increased throughput—examine multiple samples simultaneously
  • Study only your regions of interest—custom-designed primer sets
  • Assured quality—wet tested

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Access Array Workflow

  • Dispense samples. Standard 384-well plate format assures compatibility with your existing workflow.
  • Dispense primers. Only 5µl of primers and sample is used, minimizing the use of expensive reagents.
  • Load the array. The Fluidigm IFC Controller AX is used to fill the Access Array IFC in a completely automated process.
  • Thermal Cycle. Use the same thermal cycling parameters as a traditional PCR reaction—no need to re-optimize.
  • Harvest the PCR product. The IFC Controller AX automatically pools and harvests the product on a per sample basis.
  • Collect samples. Use a traditional micropipetter to collect the amplified product from each of the 48 samples.


Specifications

Key Features

InputFeaturesBenefits
 48 Samples per array 2,304 individual PCR reactions Product pooled automatically
48 Primer pairs per array
 30nL individual reaction volumeEach sample is harvested individually
 Only 96 pipette steps per array Fixed reaction volumes assure reproducibility Only 48 pipette steps to harvest product









Sequence Captures

Capture per Sample Capture Per Array
Amplicon Tagging* 24kb 1.1MB
Multiplexed Amplicon Tagging* 240kb 11MB
Long Range PCR 480kb 23MB