Fluidigm - Accelerate and Enable

SNP Genotyping

48.48 Dynamic Array

96.96 Dynamic Array

EP1 System

BioMark™ System

EP1 System

Stand-Alone Thermal Cycler

IFC Controller

SA Thermal Cycler

   
   
Dynamic Array
  384-well 96.96 48.48
Master Mix:  46mL 240ul 120ul
Primer- probe:  4.6mL 240ul 120ul
Plates:  24 1 4
Time:  8 days 3 hours 8 hours
Pipette steps:  18,432 192 384
Comparison of materials and pipetting steps between conventional microplates and dynamic arrays; 96 samples against 96 SNP's

Fluidigm Dynamic Array for SNP Genotyping

Bringing the density of microarrays to the world of TaqMan SNP Genotyping.

The Dynamic Array Difference

  • Setup 9,216 SNP Genoptyping reaction with 192 pipette steps
  • Use your existing TaqMan® reagents
  • Enjoy 99% conversion rates and 99% call rates

The Dynamic Array Solution

The Fluidigm Dynamic Arrays allow you to use your existing TaqMan SNP Genotyping assays in a flexible, easy-to-use and cost effective fashion. Each dynamic array allows you to setup up to 9,216 individual TaqMan reactions in a single experiment. Simply dispense 96 gDNA samples and 96 TaqMan assays and the dynamic array will then do the work of assembling the samples and reagents to create individual TaqMan reactions.

Dynamic Arrays

There are currently two versions of dynamic arrays available; the 96.96 Dynamic Array allows you to test 96 samples against 96 SNP’s. The 48.48 Dynamic Array allows you to test 48 samples against 48 SNP’s.

Click the links below to open Technical Specifications

data table data table
The Fluidigm® 96.96 Genotyping Chip at a Glance
Parameter Specification
Assay conversion rate*: 99.00%
Call accuracy: 99.75%
Call rate: 99.00%
Chip footprint: 128 mm x 85 mm x 14 mm
Inlet spacing on input frame: 4.5 mm pitch
Liquid-transfer steps: 192
Primer-probe inlets: 96
Sample inlets: 96
Reaction chambers: 9,216 (96 x 96)
Chip loading: IFC Controller HX
Detection and analysis: BioMark™ System or EP1 Reader
The Fluidigm® 48.48 Genotyping Chip at a Glance
Parameter Specification
Assay conversion rate*: 99.00%
Call accuracy: 99.75%
Call rate: 99.00%
Chip footprint: 128 mm x 85 mm x 14 mm
Inlet spacing on input frame: 4.5 mm pitch
Liquid-transfer steps: 96
Primer-probe inlets: 48
Sample inlets: 48
Reaction chambers: 2,304 (48 x 48)
Chip loading: IFC Controller MX
Detection and analysis: BioMark™ System or EP1 Reader

*Percent of licensed PCR assays that yield 99.75% accuracy on BioMark™ dynamic arrays.

 
BioMark™ Digital PCR Analysis Software screenshot
(click image to enlarge)

Global Allele Map
A 96.96 Dynamic Array produces 9,216 data points, which consist of the pair-wise combinations of every assay and every sample. The cluster diagram on the left shows 96 different genotyping assays and 94 different samples. Call rates are greater than 99.5%.

BioMark™ Digital PCR Analysis Software screenshot
(click image to enlarge)

Intra- and Inter-chip Reproducibility of Assay Clusters
[Panel A and Panel B, in duplicate]

Scatter plots for panel A or B and panels A and B visually show the reproducibility of intra- and inter-chip genotyping runs. (red and green = homozygous, blue = heterozygous)

BioMark™ Digital PCR Analysis Software screenshot
(click image to enlarge)

Concordance with Microwell Plate Systems
Genotyping calls for 12 assays and 46 samples (552 total reactions) were generated on a dynamic array and on a widely used microwell plate platform. The color-coded scatter plots (red and blue = homozygous, green = heterozygous) show 100% concordance.

Videos


Posters

January 10 - 14, 2009
Plant and Animal Genome XVII Conference: PAG 2009; San Diego, CA

Poster: High-Throughput SNP Genotyping in Salmon and Steelhead

PSDI 2008 poster, Fluidigm
Adobe pdf icon View pdf of poster

November 11 - 15, 2008
58th Annual Meeting of the American Society of Human Genetics: ASHG 2008; Philadelphia, PA

Poster: Rapid Screening of Genetic Factors Associated With Warfarin Dosage Using a Nanofluidic System
Presented by: Ramesh Ramakrishnan, PhD; Director of Molecular Biology

ASHG 2008 poster, Fluidigm
Adobe pdf icon View pdf of poster
mp3 icon Listen to the audio presentation by Dr. Ramakrishnan

November 10 - 13, 2008
qPCR Symposium; Millbrae, CA

Poster: Efficient Drug Metabolism Studies Using the BioMark™ System

BSHG 2008 poster, Fluidigm
Adobe pdf icon View pdf of poster

Fluidigm® Arrays

Product Data Sheet
The Fluidigm 96.96 Dynamic Array – SNP Genotyping – provides the flexibility of a microwell plate and the density of a microarray in one easy-to-use, consumable integrated fluidic circuit (IFC).

Product Data Sheet
The Fluidigm 48.48 Dynamic Array – SNP Genotyping – delivers a new level of efficiency for the study of genetic variation within large populations.

Application Note
Fluidigm Dynamic Arrays and SNP Genotyping Meet the Challenge of Microarray Validation

Application Note
Fluidigm System for Rapid, Reliable, and Cost Effective Genotyping.

Application Note
Efficient Drug Metabolism Studies using the Fluidigm System

BioMark™ System for Genetic Analysis

Product Data Sheet
The BioMark system streamlines workflow for applications demanding sensitivity and dynamic range, at extremely high throughput.

Product Data Sheet
The BioMark™ System Software Suite offers the perfect combination of instrument management, data analysis, and data management tools, as well as an easy-to-use software interface.

Fluidigm® EP1 System for Genetic Analysis

Product Data Sheet
The EP1 Reader opens the door to the power of integrated fluidic circuits (IFCs) for high-throughput SNP genotyping and digital PCR.

Product Data Sheet
The Fluidigm® System Software Suite offers the perfect combination of instrument management, data analysis, and data management tools, as well as an easy-to-use software interface.

Stand-Alone Thermal Cycler

Product Data Sheet
The Fluidigm Stand-Alone Thermal Cycler (STC) provides an ideal solution for end-point PCR assays on Fluidigm integrated fluidic circuits (IFCs).

IFC Controllers

Product Data Sheet
The Fluidigm IFC Controller HX allows for the easy and simple setup of high density reactions within the integrated fluidic circuit (IFC) chips.

Product Data Sheet
The Fluidigm IFC Controller MX allows the simple setup of high-density reactions within the integrated fluidic circuit (IFC) chips.

BioMark™ Bibliography

BioMark™ Bibliography
Gene Expression, Genotyping, Digital PCR - BioMark related journal publications.