The Digital Array Difference

• Obtain absolute measurement of your amplifiable sample
• Eliminate costly titration steps while improving data quality
• Use as little as 1 pg of material
  

The Power of Digital PCR

SlingShot™ sample quantification is a novel application of digital PCR for absolute quantification of amplifiable DNA in your library. With SlingShot, you gain the power of digital PCR on digital arrays to find the optimal DNA-to-bead ratio for emPCR.

SlingShot Explained

SlingShot Kit uses digital PCR assays to detect only amplifiable molecules within the sample mixture. This absolute measure eliminates the need for mass-based calibrators or reference samples. In addition, because of digital PCR sensitivity, only minimal sample concentrations are required to achieve quantitative results. The accuracy and sensitivity offered by SlingShot allows you to sequence previously undetectable samples while saving time and money.

Available SlingShot Assays

Kit	Description
BMK-SLG-454SG	454 Shotgun Library Assay Set
BMK-SLG-454MID	454 MID Library Assay Set
BMK-SLG-SOL	Solexa Library Assay Set
BMK-SLG-SLD	SOLiD Library Assay Set

Sample Quantitation

All current next-generation platforms rely on the ability to amplify individual library DNA molecules, it is this ability to amplify and then to read individual DNA molecules that allows massive throughput capabilities. The challenge to instrument operators is how to accurately and reproducibly determine library concentrations which will allow them to achieve single molecule amplification.

In this example the 454 workflow is used. If the concentration of the sample is overestimated the majority of the beads will be blanked resulting in a failed sequencing read. Conversely, if sample concentration is underestimated, the majority of the beads will contain multiple copies of DNA which will in turn cause a failed read. Sequencing throughput and data quality is maximized when the maximum number of emPCR beads contain exactly one DNA molecule.

 

Videos

News

• Professor sequences his entire genome at low cost, with small team
  Bioengineering professor Stephen Quake reports sequencing his own genome for less than $50,000 and with a team of just two other people. [Editor's Note: The SlingShot™ Kit from Fluidigm was used in this project to quantitate samples for sequencing]

Overviews

• BioMark System for Genetic Analysis
• Fluidigm EP1 System for Genetic Analysis
• Fludigm Digital Arrays

Presentation

• Fluidigm SlingShot Kit