Each software application is designed to be intuitive and easy-to-use, with the industry’s best graphical user interface. The Fluidigm software features include pipetting templates, easy import/export functionality, predefined experiment layouts, and multiple data viewing options.
The SINGuLAR™ Analysis Toolset
The SINGuLAR™ Analysis Toolset is a powerful analysis tool based on the programming language R, for analyzing and visualizing single-cell gene expression data. It includes:
- Compare gene expression levels—Convert Ct values to Log2 gene expression using LoD method to assign unexpressed genes
- Sort and filter function—Sort by gene expression and rapidly exclude low variance genes
- Scalable computing—Process individual or multiple data sets in parallel
- Multivariant analysis—Analyze data in hierarchical clustering or PCA to compare factors
- Visualization tools—View data in violin plot, scatter plot, or heat map to rapidly identify major classes of individual cells
Download the scripts and sample data
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Visualization Tools - Violin Plot
Results are easily viewed in multiple plots or map views. |
Real-Time PCR Analysis
The Fluidigm Real-Time PCR Analysis software includes:
- Standard curve view—Easily calculate the standard curve by identifying dilutions samples
- Delta delta Ct calculation—Get delta delta Ct in just three easy steps
- Automated threshold setting—Automatically determine the ideal threshold setting for each chip run
- Automatic curve quality score—Quickly and efficiently sort through real-time PCR curves and exclude those that do not meet your
criteria
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Table and Amplification Curve Views
Results are easily viewed in table and amplification curve formats. |
Genotyping Analysis The Fluidigm Genotyping Analysis software includes additional features specific to genotyping applications including:
- Automatic cluster calling—Automatically increase accuracy while processing more using a highly accurate cluster-calling algorithm that ranks among the best in the industry
- Multiple chip scoring—Combines and scores multiple experiments to decrease the analysis time and improve scoring accuracy for rare
genotypes
- Confidence score—Automatically determines the confidence level of cluster calls
- Real time or end point—View real-time data that aids in assay design while using end-point imaging for high-throughput applications
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Allele Map
Automatic cluster calling results are easily viewed in multiple formats. |
Digital PCR Analysis
The Fluidigm Digital PCR software includes features specific to digital PCR, including:
- Real-time digital PCR—Use real-time analysis to confirm specificity of digital PCR
- Multiplex capability—Quantitate up to four targets per sample
in each reaction by using up to four reporter dyes
- Copy number variation—Automatically apply statistical correction and determine the ratio of your targets of interest
- End-point analysis—Use end-point image for analysis, greatly increasing the throughput of the system
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Heat Map and Call/No-Call Map
Automatic counts and reports for the number of positives for each reporter dye are easily viewed in multiple formats.
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