Dynamic Array™ IFC

Fluidigm Dynamic Array integrated fluidic circuits (IFCs) deliver a new level of efficiency and throughput for real time PCR and genotyping applications. Dynamic Array IFCs have an on-chip network of microfluidic channels, chambers, and valves that automatically assemble individual PCR reactions, decreasing the number of pipetting steps required by up to 100 fold. Reusable chip formats have also been developed to dramatically decrease costs and increase throughput. The available chip formats are:

• 192.24 - 192 samples x 24 assays (single use)
• FR48.48 – 48 samples x 48 assays (reusable, SNP Genotyping use only)
  
• 48.48 – 48 samples x 48 assays (single use)
  
• 96.96 – 96 samples x 96 assays (single use)
  

The 96.96 Dynamic Array IFC

Digital Array™ IFC

The Fluidigm Digital Array integrated fluidic circuit (IFC) enables users to conduct digital PCR experiments. The digital array partitions a sample premixed with PCR reagents into hundreds of individual PCR reactions. This partitioning of the mixture allows the absolute quantification of target sequences. Digital PCR is a novel technique for PCR applications demanding extreme accuracy of copy number variation.

Using the Fluidigm Digital PCR technology, there are just five easy steps from setup to analysis to discovery.  See how it's done.

The 48.770 Digital Array IFC

Access Array™ IFC

The Access Array IFC is the first commercially available microfluidic device which allows the user to recover the PCR product. It automatically assembles 2,304 unique PCR reactions, each reaction a combination of one of the 48 samples and one of the 48 primer inlets. Post PCR the product of 48 reactions per sample is harvested out of the Access Array IFC for downstream sequencing.

Protein Crystallization IFCs

TOPAZ IFC chips provide the most efficient screen-to-beam solution for protein crystallization. Here are a just a few of the advantages of TOPAZ chips:

• Free Interface Diffusion (FID) and dehydration based experiments
• Ultra low sample volume requirements
• Simple, easy to use workflow for setting up experiments

TOPAZ 1.96 Diffraction Capable IFC

Slingshot™ Kit

The SlingShot Sample Quantification Kit provides is a novel application of digital PCR for absolute quantification of amplifiable DNA in your library. With the SlingShot kit, you gain the power of digital PCR on digital arrays for finding the optimal DNA-to-bead ratio for emPCR.

The SlingShot Kit uses digital PCR assays to detect only amplifiable molecules within the sample mixture. This absolute measurement eliminates the need for mass-based calibrators or reference samples. In addition, because of the sensitivity of digital PCR, only minimal sample concentrations are required to achieve quantitative results. The accuracy and sensitivity offered by SlingShot technology allows you to sequence previously undetectable samples while saving time and money.

Key Benefits:

• Obtain absolute measurement of your amplifiable sample
• Eliminate costly titration steps while improving data quality
• Use as little as 1 pg of material

Each bright spot indicates a positive PCR reaction or an amplicon that can be sequenced. By counting the number of positive reactions the total copy number of amplicons in the samples of interest can be determined.

EGFR-Met Panel

The EGFR-Met panel is optimized to work with the Access Array System enabling users to quickly and easily resequence the EGFR and Met gene exons. The panel is ideal for any researcher interested in lung cancer research.