Setting the new standard for high resolution CNV studiesCopy Number Variations (CNVs) are important for understanding the differences in genetic variation and possible association with various diseases. Current techniques are limited in sample throughput and the resolution required to extensively study these variations in depth. Fluidigm Digital Array™ integrated fluidic circuits (IFCs) offer the solution for this challenge.
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A new level of sensitivity and flexibility in detecting copy number variations is made possible using Digital Array integrated fluidic circuits (IFCs) and the power of digital PCR. Digital PCR is an extremely accurate method of quantifying a sample and is used to accurately determine the CNVs in a given sample. The copy number determination is made by simultaneous quantification of both the gene of interest and a reference gene by using a different fluorescent dye for each assay. The ratio of the counts obtained with the two dyes is the relative copy number of the gene of interest in the sample. This can be accomplished in a single panel on the Digital Array IFC to determine the difference between four and five copies or across multiple panels for even greater resolution. |
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Systems |
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Opens the door to the power of integrated fluidic circuits (IFCs) for digital PCR. |
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Streamlines workflow for applications demanding sensitivity and dynamic range, at extremely high throughput. |
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Chips
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Digital Array™ IFCs Digital PCR for absolute quantitation of molecular targets. |
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