2424 Fluidigm Application: Gene Expression
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Gene Expression

Bringing the density of microarrays to the world of qPCR

Scientists studying gene expression have previously needed to study one or two genes at a time or perform a larger scale study on microarrays. The BioMark™ System enables microarray-like studies using qPCR by enabling the easy setup of more than 9,000 qPCR reactions, using reagents you already have in your lab.

Key benefits include:

  • High throughput and low cost, using only a fraction of the pipette steps necessary for other systems
  • Compatibility with your existing reagents, such as TaqMan® assays
  • Data quality consistent with TaqMan standards
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Gene Expression

The BioMark™ System is ideal for mid- to high-throughput targeted gene expression studies. The BioMark System supports many commercially available TaqMan® gene expression assays, as well as assays detected with EvaGreen® dye, and those designed for miRNA analysis.

The BioMark System -- based on proprietary microfluidic chips called integrated fluidic circuits (IFCs) -- provides high-throughput real-time qPCR, enabling researchers to streamline their experimental workflow, increase throughput, and lower costs. The workflow is simple: samples and assays are placed on the Dynamic Array IFC and mixed using an IFC Controller. The Dynamic Array IFC is then run on the BioMark System for real-time PCR and data collection. Since nanoliter reaction volumes require less reagent than standard plate PCR, the BioMark System saves both time and money for the high-throughput real-time PCR study. With the BioMark System, one technician can run 30,000 PCR reactions in a single day.

Fluidigm DELTAgene Assays are high-quality, rapid turnaround, custom qPCR gene expression assays. They permit you to take full advantage of your BioMark System with minimal experiment setup time and validated protocols that provide quality results.

System

 

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BioMark™HD System

Streamlines workflow for applications demanding sensitivity and dynamic range, at extremely high throughput.


Chips



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Dynamic Array™ IFCs

Maximize efficiency for Gene Expression Analysis. Options: 192.24, 96.96, 48.48 


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