Evaluation of Nanofluidics Technology for High-Throughput SNP Genotyping in a Clinical Setting
There is a need for high-throughput genotyping platforms for targeted validation of disease-associated single nucleotide polymorphisms (SNPs). Researchers used DNA extracted from peripheral blood and buccal wash samples to evaluate the use of microfluidic technology versus TaqMan® with traditional sequencing.
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Single-cell gene expression profiling reveals qualitatively distinct CD8 T cells elicited by different gene-based vaccines
Understanding the mechanism of protective immunity mediated by CDS T cells is dependent upon the definition of heterogeneity, and understanding of immune responses generated by different vaccines. Researchers identified previously unrecognized subsets of CD8 T cells with gene expression patterns of single cells and showed that they are differentially induced by different vaccines.
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Fluidigm Assays and Target-specific Primers for gene expression, genotyping and targeted resequencing
Fluidigm now offers services to deliver gene expression and genotyping assays as well as target-specific primers for amplification and tagging of next-generation sequencing samples.
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192.24 Dynamic Array™ Integrated Fluidic Circuit (IFC)
The Fluidigm 192.24 Dynamic Array Integrated Fluidic Circuit (IFC)
provides the ideal solution for high sample throughput SNP genotyping.
It is designed to genotype 192 samples against 24 SNP assays in a
single run and incorporates innovative design features to greatly
increase sample throughput, provide outstanding data quality, and an
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Fluidigm and BD Biosciences - Single-Cell Isolation and Analysis
Variation in phenotype and gene expression at the single-cell level may be pivotal in the differentiation of stem cells. Here, BD Biosciences and Fluidigm present a protocol to discriminate different HSC subtypes on the basis of Hoechst dye efflux and SPKLS surface staining. Single cells from the Ly-HSC and My-HSC subpopulations were isolated using a BD FACSAria™ III flow cytometer and differences in gene expression between the two populations were determined using the BioMark™ System. The results demonstrate that the combination of flow cytometry and single-cell gene expression provides a powerful research tool. This combination provides a simple and robust method of both single-cell isolation and analysis of hematopoietic development.