Targeted polymerase chain reaction-based enrichment and next generation sequencing for diagnostic testing of congenital disorders of glycosylation. Genet Med, August 1, 2011
Using next-generation sequencing, researchers identify disease-causing mutations associated with congenital disorders of glycosylation. Development and validation of a sequencing panel to test 24 genes resulted in identification of positive controls and a number of variants.
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Going to be in Montreal for ASHG/ICHG - October 11-15, 2011?
Learn how your targeted resequencing can be as easy as 1-2-3.
Join us for a workshop at ASHG/ICHG featuring Joseph Boland, NCI; Henry Erlich, Roche Molecular Systems; and Martin Pieprzyk, Fluidigm Corporation. learn more about the Access Array™ System - the only technology that offers a complete upfront solution for targeted resequencing on all next-generation sequencing platforms.
Single-Cell Gene Expression
Induction of human neuronal cells by defined transcription factors.
Nature, May 26, 2011
A team of researchers has discovered how to induce non-neuronal cells into becoming functional neurons with classic neuronal morphologies, protein markers, gene expression, and action potentials. Their data demonstrate that non-neuronal human somatic cells, as well as pluripotent stem cells, can be converted directly into neurons by insertion of neuronal transcription factors. These methods may facilitate robust generation of person-specific human neurons for in vitro disease modeling or future applications in regenerative medicine.
MicroRNA-mediated conversion of human
fibroblasts to neurons.
Nature, July 13, 2011
Researchers studied the transcription factors and signalling pathways that aid the formation of neurons. They discovered that miR-9/9 and miR-124 are involved in the induction of neuronal morphology. Using single-cell gene expression, they were able to characterize neurons in these mRNAs and further study their role in human neurogenesis.
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Visit the following web pages to learn how Fluidigm technology is enabling researchers by providing systems and consumables that provide robust results while saving time and money.
*IFC = Integrated Fluidic Circuit