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Next Gen Sequencing

48.770 Digital Array 12.765 Digital Array

48.770 Digital Array

12.765 Digital Array

BioMark System EP1 Reader

BioMark System

EP1 System

   
SlingShot data

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Each bright spot indicates a positive PCR reaction or an amplicon that can be sequenced. By counting the number of positive reactions the total copy number of amplicons in the samples of interest can be determined.

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Sample Quantification for DNA Sequencing

SlingShot™ Kit from Fluidigm

Bringing the power of Digital PCR to next generation sequencing

The Digital Array Difference

  • Obtain absolute measurement of your amplifiable sample
  • Eliminate costly titration steps while improving data quality
  • Use as little as 1 pg of material

The Power of Digital PCR

SlingShot™ sample quantification is a novel application of digital PCR for absolute quantification of amplifiable DNA in your library. With SlingShot, you gain the power of digital PCR on digital arrays to find the optimal DNA-to-bead ratio for emPCR.

SlingShot Explained

SlingShot Kit uses digital PCR assays to detect only amplifiable molecules within the sample mixture. This absolute measure eliminates the need for mass-based calibrators or reference samples. In addition, because of digital PCR sensitivity, only minimal sample concentrations are required to achieve quantitative results. The accuracy and sensitivity offered by SlingShot allows you to sequence previously undetectable samples while saving time and money.

Available SlingShot Assays

Kit Description
BMK-SLG-454SG 454 Shotgun Library Assay Set
BMK-SLG-454MID 454 MID Library Assay Set
BMK-SLG-SOL Solexa Library Assay Set
BMK-SLG-SLD SOLiD Library Assay Set

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Fluidigm SlingShot Kit