25cf Fluidigm Applications Sample Quantitation for Sequencing
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Sample Quantitation for Sequencing

Bringing the power of IFCs to Next-Generation Sequencing

Next-generation sequencing promises to revolutionize life science in fields as diverse as human genomics, species discovery, and personalized medicine.  Despite this cost effectiveness and throughput capability, the technology faces barriers to widespread adoption, among them the complexity of sample preparation and difficulty of use. The SlingShot™ kit overcomes some of these challenges by allowing users to quickly and easily determine the absolute concentration of their library, prior to sequencing to ensure ideal sequencing results.

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Sample Quantitation

Current next-generation sequencers rely on the ability to amplify individual library DNA molecules either through emPCR or bridge amplification. It is this single-molecule amplification capability that allows the sequencers to generate enough copies of individual molecules to be read during the sequencing run. The success of the entire single molecule amplification process relies on the sequencer's ability to amplify individual molecules, and the instrument operator's ability to accurately and reproducibly determine library concentrations.



Key Feature: Determination of Library Concentration

In this example the 454 workflow is used. If the concentration of the sample is overestimated the majority of the beads will be blanked resulting in a failed sequencing read. Conversely, if sample concentration is underestimated, the majority of the beads will contain multiple copies of DNA which will cause a failed read. Sequencing throughput and data quality is maximized when the maximum number of emPCR beads contain exactly one DNA molecule.


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Systems

 

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EP1™ System

Opens the door to the power of integrated fluidic circuits (IFCs) for digital PCR.


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BioMark™ HD System

Streamlines workflow for applications demanding sensitivity and dynamic range, at extremely high throughput.


Chips



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Digital Array™ IFCs

Digital PCR for absolute quantitation of molecular targets.


 



 

 


Specification Sheet




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