|Key Application: SNP Genotyping
Fluidigm SNPtype Assays provide a custom, high- throughput, low-cost
single nucleotide polymorphism (SNP) genotyping solution which enables
rapid assay design and polymorphism screening. The assays are based on
allele-specific PCR SNP detection chemistry and combine the advantages
of minimum experimental setup time and flexible assay choice with the
reliability of Dynamic Array™ integrated fluidic circuits (IFCs).
Low costs and high reproducibility are critical for high sample throughput applications including production-scale SNP genotyping and screening studies. SNPtype Assays employ tagged, allele-specific PCR primers and a common reverse primer. A universal probe set is used in every reaction, producing uniform fluorescence while significantly reducing both startup and running costs. The assays provide excellent results on Dynamic Array IFCs and are compatible with existing workflows. We provide the locus-specific primer sequences with all orders, confirming target locations and assuring reproducibility between laboratories.
Call map view for 48 cattle samples and 48 SNPtype Assays; cluster plot for a typical SNPtype Assay