Area of Interest Genomics Analysis NGS Library Prep Targeted Sequencing Targeted Sequencing with Microfluidics

Targeted DNA Seq 

Achieve economy of scale with ease 

Reduce hands-on time and drive down reagent consumption with automated microfluidics, resulting in significant savings. And to ensure that your NGS panel always remains up-to-date, maintain flexible customization capabilities unique to Fluidigm. 

Blue icon illustrating targeted sequencing application

SPOTLIGHT

 

DATA SHEET | Juno Targeted DNA Sequencing Library Preparation System 

Customized assay panels in convenient kits provide investigators and genomics service providers with an easily scalable and automated solution. Targeted DNA Sequencing Library Preparation is compatible with Illumina® MiniSeq™, MiSeq™, NextSeq™ and HiSeq® NGS systems. 

FEATURE | Advanta CFTR NGS Library Prep Assay saves time and money through an efficient and scalable targeted NGS workflow 

The Advanta™ CFTR NGS Library Prep Assay automates targeted enrichment of cystic fibrosis transmembrane conductance regulator (CFTR) variants from each of the gene’s 27 exons and select intronic regions using proven microfluidics technology. The workflow significantly lowers hands-on time while conserving precious reagents by reducing reactions to the nanoliter scale. With the capacity to produce from 48 to over 1,500 NGS libraries per week, scalability is simplified to meet laboratory needs as they change over time. Protocols are available that support Juno™ and Access Array™ systems. 

Advanta Evidence-Based CFTR Variant Coverage Flyer DOWNLOAD 

Methods 

Juno Targeted DNA Sequencing Library Preparation 

Scale with the industry’s most flexible per-sample throughput and enrichment capabilities using the Juno targeted DNA Sequencing Library Preparation System. Two sample throughput options are available, LP 48.48 and Juno LP 192.24 IFC (integrated fluidic circuit). 

The LP 192.24 IFC automates amplicon-based libraries for 192 unique DNA samples per run and up to 2,400 amplicon targets per sample. The LP 48.48 IFC accommodates up to 48 DNA samples per run and up to 4,800 amplicon targets per sample. Targeted DNA Sequencing Library Preparation is fully compatible with all Illumina® sequencing systems. Up to 1,536 sample libraries may be multiplexed per sequencing run. 

Access Array Library Preparation 

Access Array™ delivers specimen flexibility supporting sample types of DNA extracted from germline and FFPE somatic samples. Access Array automates targeted DNA library preparation for 48 unique DNA samples per run and up to 4,800 amplicon targets per sample using Targeted DNA Seq Library reagents, or up to 480 amplicon targets per sample using Access Array chemistry. 

The system is compatible with multiple sequencing platforms including Illumina® sequencing systems and, for use with Access Array chemistry, Ion Torrent™. 

Publications

Powering over 1,500 scientific publications with our products 

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Dr. Dexi Chen, PhD, MD

Spotlight IMC DR. DEXI CHEN

Achieving multi-omic insights with microfluidics and CyTOF technology

Jo Vandesompele, PhD

Spotlight MF Jo Vandesompele

Jo Vandesompele at Ghent University discusses a deep dive into single-cell sequencing

Christophe Lancrin, PhD

Spotlight MF Christophe Lancrin

Christophe Lancrin on revealing new transcription factors using C1 and Biomark

Ansuman Satpathy, MD, PhD

Spotlight MF Ansu Satpathy

Stanford’s Ansu Satpathy designed single-cell T-ATAC-seq, now available at Script Hub

Shyam Prabhakar, PhD

Spotlight MC SHYAM PRABHAKAR

Shyam Prabhakar explores signatures of patient survival and EMT in colorectal tumors

Simone Mayer, PhD

Spotlight MF Simone Mayer

Simone Mayer, PhD, explains how using multimodal analysis helped reveal new insights

Kelly Nudelman, PhD

Spotlight MF Kelly Nudelman and Tae-Hwi Schwantes

Kelly Nudelman and Tae-Hwi Schwantes-An discuss sample identification

Shyam Prabhakar, PhD

Spotlight MC SHYAM PRABHAKAR

Shyam Prabhakar explores signatures of patient survival and EMT in colorectal tumors

Jonathan M. Irish, PhD

Combinatorial, single-cell approaches to explore the complexity of biologic systems

Scott Magness, PhD

Spotlight MF SCOTT MAGNESS

Investigating transcriptomic changes at the microscale level

Dr. Luc Teyton, MD, PhD

Spotlight MC DR. LUC TEYTON

How one lab explores the immune system one T cell at a time

Scientist Michael Stubbington, PhD

A new single-cell sequencing tool brings fresh insight to functional T cell studies

Customer stories

Learn more about how our customers are leveraging Fluidigm technology

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Unless explicitly and expressly stated otherwise, all Fluidigm products are provided for Research Use Only, not for use in diagnostic procedures. For more information see www.fluidigm.com/legal/notices