Area of Interest Genomics Analysis PCR Applications Gene Expression with Microfluidics

Gene Expression 

Advance your translational and clinical research with TaqMan or Delta Gene assays 

Data reproducibility is key to differential gene expression studies. The scalable Biomark™ HD and Biomark X systems supports tens to hundreds of samples at nanoliter volumes. Combining microfluidics technology with optimized reagents produces high-quality results in less time. Ideal for labs performing hundreds to thousands of samples, the Biomark HD system provides significant cost and time advantages over plate-based technologies. And with a wide range of integrated fluidic circuit (IFC) formats available, scaling from mid- to high-throughput on a single system is easily achievable. 

Blue icon illustrating gene expression application

Methods 

Delta Gene

Pair Delta Gene™ assays and reagents with EvaGreen® dye for an economical alternative to probe-based detection. EvaGreen® binds to double-stranded DNA, resulting in fluorescein-like fluorescence excitation and emission spectra. EvaGreen® also minimizes PCR inhibition compared to SYBR® Green and other nucleic acid stains. 

TaqMan

Probe-based assays use primers to detect sequence-specific single-cell cDNA probes. The method enhances qPCR through dual-labeled hydrolysis probes and PCR primers. Specific target detection requires binding three independent oligonucleotides, which reduces fluorescence from nonspecific sources. Our chemistries work seamlessly with your existing TaqMan® assay library to support both standard and fast protocols. 

Publications

Powering over 1,500 scientific publications with our products

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Dr. Dexi Chen, PhD, MD

Spotlight IMC DR. DEXI CHEN

Achieving multi-omic insights with microfluidics and CyTOF technology

Jo Vandesompele, PhD

Spotlight MF Jo Vandesompele

Jo Vandesompele at Ghent University discusses a deep dive into single-cell sequencing

Christophe Lancrin, PhD

Spotlight MF Christophe Lancrin

Christophe Lancrin on revealing new transcription factors using C1 and Biomark

Ansuman Satpathy, MD, PhD

Spotlight MF Ansu Satpathy

Stanford’s Ansu Satpathy designed single-cell T-ATAC-seq, now available at Script Hub

Shyam Prabhakar, PhD

Spotlight MC SHYAM PRABHAKAR

Shyam Prabhakar explores signatures of patient survival and EMT in colorectal tumors

Simone Mayer, PhD

Spotlight MF Simone Mayer

Simone Mayer, PhD, explains how using multimodal analysis helped reveal new insights

Kelly Nudelman, PhD

Spotlight MF Kelly Nudelman and Tae-Hwi Schwantes

Kelly Nudelman and Tae-Hwi Schwantes-An discuss sample identification

Shyam Prabhakar, PhD

Spotlight MC SHYAM PRABHAKAR

Shyam Prabhakar explores signatures of patient survival and EMT in colorectal tumors

Jonathan M. Irish, PhD

Combinatorial, single-cell approaches to explore the complexity of biologic systems

Scott Magness, PhD

Spotlight MF SCOTT MAGNESS

Investigating transcriptomic changes at the microscale level

Dr. Luc Teyton, MD, PhD

Spotlight MC DR. LUC TEYTON

How one lab explores the immune system one T cell at a time

Scientist Michael Stubbington, PhD

A new single-cell sequencing tool brings fresh insight to functional T cell studies

Customer Stories

Learn more about how our customers are leveraging Fluidigm technology.

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Unless explicitly and expressly stated otherwise, all Fluidigm products are provided for Research Use Only, not for use in diagnostic procedures. For more information see www.fluidigm.com/legal/notices