CyTOF Mass Cytometry News

Catch up on 2015 publications, reagent releases and mass cytometry meetups

Mass cytometry use has grown steadily since CyTOF appeared in 2008. Fluidigm provides an end-to-end solution combining data analysis, instrumentation, reagents and a comprehensive catalog of preconjugated antibodies for high-parameter single-cell protein research. Partner with us to empower your lab.

2015 publications

Researchers are answering questions that hadn’t even been imagined previously. Scroll down for the most recent 2015 results published by CyTOF users making discoveries at the functional protein level.

CyTOF has played a key role in more than a dozen published papers already this year, in areas including dermatology, arthritis, pregnancy, cancer, stem cells and immunology.

In addition to the cell type discoveries and functional profiling described in these publications, several papers present new methods in high-dimensional data analysis or sample barcoding approaches. See the most recent publications below, or read the full publications list.

Notable research

Brodin, P., Jojic, V., Gao, T. et al. “Variation in the human immune system is largely driven by non-heritable influences.” Cell 160 (2015): 37–47.

Han, L., Qiu, P., Zeng, Z. et al. “Single-cell mass cytometry reveals intracellular survival/proliferative signaling in FLT3-ITD-mutated AML stem/progenitor cells.” Cytometry A 87 (2015): 346–356.

Hansmann, L., Blum, L., Ju, C. et al. “Mass cytometry analysis shows that a novel memory phenotype B cell is expanded in multiple myeloma.” Cancer Immunology Research (2015). doi: 10.1158/2326–6066

Kay, A.W., Bayless, N.L., Fukuyama J. et al. “Pregnancy does not attenuate the antibody or plasmablast response to inactivated influenza vaccine.” Journal of Infectious Diseases (2015). doi: 10.1093/infdis/jiv138

Lujan, E., Zunder, E.R., Ng, Y.H. et al. “Early reprogramming regulators identified by prospective isolation and mass cytometry.” Nature (2015). doi:10.1038/nature14274

Miner, J.J., Aw-Yeang, H., Fox, J.M. et al. “Chikungunya viral arthritis in the United States: a mimic of seronegative rheumatoid arthritis.” Arthritis and Rheumatology (2015). doi: 10.1002/art.39027

Watanabe, R., Gehad, A., Yang, C. et al. “Human skin is protected by four functionally and phenotypically discrete populations of resident and recirculating memory T cells.” Science Translational Medicine 7 (2015): 279ra39.

Zunder, E.R., Lujan, E., Goltsev, Y. et al. “A continuous molecular roadmap to iPSC reprogramming through progression analysis of single-cell mass cytometry.” Cell Stem Cell 16 (2015): 323–337.

Methods

Lai, L., Ong, R., Li, J. et al. “A CD45-based barcoding approach to multiplex mass-cytometry (CyTOF).” Cytometry A 87 (2015): 369–374.

Mei, H.E., Leipold, M.D., Schulz, A.R. et al. “Barcoding of live human peripheral blood mononuclear cells for multiplexed mass cytometry.” Journal of Immunology 194 (2015): 2022–2031.

Tricot, S., Meyrand, M., Sammicheli, C. et al. “Evaluating the efficiency of isotope transmission for improved panel design and a comparison of the detection sensitivities of mass cytometer instruments.” Cytometry A 87 (2015): 357–368.

Van Gassen, S., Callebaut, B., Van Helden, M.J. et al. “FlowSOM: Using self-organizing maps for visualization and interpretation of cytometry data.” Cytometry A (2015). doi: 10.1002/cyto.a.22625

Zunder, E.R., Finck, R., Behbehani, G.K. et al. “Palladium-based mass tag cell barcoding with a doublet-filtering scheme and single-cell deconvolution algorithm.” Nature Protocols 10 (2015): 316–333.

Congratulations Bernd Bodenmiller

Professor Bernd Bodenmiller of the University of Zurich was awarded the 2015 Robert J. Cotter New Investigator Award by the US Human Proteome Organization (US HUPO). His team is using mass cytometry to unravel the development of cancerous cell-to-cell signaling network interactions in the tumor microenvironment.

Spotlight: Immune Signatures Predict Surgery Recovery

Stanford’s Brice Gaudillière, MD, PhD, is doing groundbreaking research in collaboration with Gabriela Fragiadakis, Robert Bruggner, Martin Angst and Garry Nolan to reveal how activation of specific cell transcription factors correlates with the speed of recovery after surgery. Watch the video to learn more about this researcher on the fast track to developing an immune system fitness test.

Spotlight: Passion, Imagination & CyTOF

Sean Bendall, PhD, is advancing proteomics research at Stanford using mass cytometry to expand insights into human biology. His group is focusing on drug and signaling responses in the developing immune system. Read about this entirely new approach to scientific workflows.

Upcoming conferences

If you’re planning to attend these scientific meetings, join us to learn how mass cytometry can advance your research. If you will be presenting posters or talks, email us at support.northamerica@fluidigm.com.

Immunology 2015™

May 8–12, New Orleans, booth #441. Mass cytometry–related posters and presentations include:

Authors Title Time Location

Gaudillière et al.

Pre-operative immune signatures correlate with recovery from surgical trauma (HUM1P.267)

Saturday, May 9
2:30–3:45 pm

52.16 Exhibit/Poster Hall

Kleinsteuber et al.

Characterization of functional antiviral CD8 T cell immune responses of HIV elite controllers by high-dimensional phenotyping using mass cytometry (VIR6P.1169)

Sunday, May 10
2:30–3:45 pm

149.09 Exhibit/Poster Hall

Bandura et al.

Enhancements in mass cytometry for multiplexing samples and increasing cell detection rate (TECH3P.938)

Monday, May 11
2:30–3:45 pm

207.08 Exhibit/Poster Hall

Ornatsky et al.

Metal-conjugated neutravidin for MHC multimer assays using mass cytometry (TECH3P.939)

Monday, May 11
2:30–3:45 pm

207.09 Exhibit/Poster Hall

CyTOF User Meeting

Fluidigm is hosting the annual CyTOF User Meeting on Thursday afternoon, June 25 in Glasgow, Scotland, the day before CYTO® 2015 begins. Join fellow CyTOF enthusiasts for an enlightening day of insights and ideas. More details are on the way so save the date.

CYTO 2015

June 26–30, Glasgow, Scotland, booth #311.

Product highlights

See what’s new to help your lab generate data faster in the quest to reveal biological systems drivers. Visit Helios to learn more, or contact us.

CyTOF® reagents

To accelerate your research, our Maxpar® catalog features dozens of off-the-shelf metal-conjugated antibody additions for 2015: 31 anti-human, 14 anti-mouse and 6 cross-reactive antibodies. Review the newest Maxpar metal-conjugated antibody selections, or review the full Maxpar catalog.

Preassembled Sample Capillary Kit

To speed setup, we have released a ready-to-use Preassembled Sample Capillary Kit (Catalog No. 105922). The legacy Sample Capillary Kit (Catalog No. 101519) is still available, and it contains all the parts for assembling a capillary. Both kits offer top-quality data and performance.

Maxpar panel designer

An interactive web application to simplify and optimize panel design, the Maxpar panel designer uses metal-conjugated antibodies from the Fluidigm catalog and custom conjugates to calculate predicted signal overlap for each panel. The tool also provides a visualization and algorithm for optimizing each target's metal tag selections for peak performance. Save, export and share panels with collaborators and import reports with CyTOF instrument software. To access Maxpar antibodies, select Design in the Fluidigm custom assay application.