How differences in detecting photons and ions impact experimental considerations in cytometry
In traditional fluorescence flow cytometry (FFC), the reporter used is a fluorescent molecule that is measured using a photomultiplier tube (PMT) or more recently with avalanche photodiodes (APDs). Mass cytometry, or CyTOF® technology, is a relatively new single-cell analysis platform with which cellular targets are labeled with metal-tagged antibodies and detected and quantified by time-of-flight mass spectrometry.
For each method, specific issues related to the detection system must be considered during this process.
From this webinar, you will learn how to improve panel design for both FFC and mass cytometry by understanding:
- Brightness and sensitivity as a measure of reporter intensity
- The impact of target abundance when pairing antigen and reporter
- How to factor in cost and sample recovery when choosing between these two techniques
- The true impact of acquisition speed on data quality
- The similarities in data analysis and application of proper controls
About the presenter:
Tim Bushnell PhD, MBA
Co-Founder Expert Cytometry
Contact us to learn more about mass cytometry.
For Research Use Only. Not for use in diagnostic procedures.