Linking T Cell Clonotype to Phenotype Webinar

Single-Cell TCR Sequencing on C1 with mRNA Seq

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T cells recognize specific antigens via T cell receptors (TCRs), an extremely diverse set of cell surface proteins produced by the rearrangement of germline V(D)J gene segments during T cell development.

In the April 2016 issue of Nature Methods, scientists at the European Molecular Biology Laboratory European Bioinformatics Institute (EMBL-EBI) and the Wellcome Trust Sanger Institute (WTSI) published a new approach for single-cell TCR sequencing—an approach that can bring new insights about the response to immunotherapies and cancer vaccines, autoimmune disease, allergy, and vaccine design and efficacy.

Using Fluidigm® single-cell whole transcriptome RNA sequencing on the C1™ system and a novel bioinformatics tool, these researchers reconstructed the full-length, paired TCR sequences from mouse T cells. In this webinar, Michael Stubbington presents TraCeR, a novel bioinformatics tool developed to identify the single-cell TCR sequences, along with graphing functions enabling correlation of clonotype and phenotype for each T cell. Tapio Lönnberg demonstrates the power of TraCeR analysis in the context of T cell fate decisions.

 Michael Stubbington Michael Stubbington, PhD
Senior Staff Scientist
Wellcome Trust Sanger Institute
 Tapio Lonnberg Tapio Lönnberg, PhD
Postdoctoral Fellow, EMBL-EBI
Visiting Scientist at Wellcome Trust Sanger Institute

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Sanger Institute