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Tra-1-60 Staining

Cell Surface Markers

Cell Biology
Research & Development, Fluidigm Corporation
0.0
2

(...) Published on Rev B

  • supported ifcs:
    Open App IFC
  • number of ifc runs:
    10

Overview

This script enables cell surface antigen staining using antibodies that are directly coupled to fluorophores to distinguish cell stemness phenotypes in situ. The example protocol is taken from the tech note entitled "Automated Cell Staining of Induced Pluripotent Stem Cells on the C1 Single-Cell Auto Prep System," which uses the pluripotency marker Tra-1-60 on iPS cells, but the protocol is extensible to other cell surface markers on other cells. Once the cells have been stained, they can be visualized on an inverted microscope. You can then proceed to a chemistry step of your choosing. This script has been updated to be compatible with all versions of the C1 Open App IFCs, including the redesigned medium 96 cell IFC.

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Protocol: Cell Stain by Tra-1-60Duration (H:M): 1:00

Cell Load and Stain

view protocol worksheet

Tested Primary Cells or Cell Lines

Cell Name Cell Type Source
Human Induced Pluripotent Stem Cell Stem Cell Cell line
Human Neural Progenitor Cell Neural Cell Cell line

Performance

This protocol used anti-Tra-1-60 (Stemgent) coupled to DyLight 488 staining to distinguish pluripotent cells from differentiated cells on the C1. It was tested on iPS (which it stains) and NPC (which it does not). A counter stain of CellTracker Orange was used to identify live cells in this protocol. It performs as expected, staining pluripotent cells, and correlates to the expected single-cell gene expression for stemness.

Publications or Articles

Performance data

Not available

Additional documents

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