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SMART Seq2

mRNA Sequencing

Karene Argoud & Esther Mellado
Oxford Genomics Centre, Wellcome Trust Centre for Human Genetics
0.0
1

(...) Published on Rev C

  • supported ifcs:
    Open App IFC
  • number of ifc runs:
    38

Overview

Modification of Picelli et al Nature Methods 2014. Protocol modified from plate based method to ensure performance on C1. This script has been updated to be compatible with all versions of the C1 Open App IFCs, including the redesigned medium 96 cell IFC.

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Protocol: Smart-seq2Duration (H:M): 2:00

Cell Load

Sample Prep

view protocol worksheet

Tested Primary Cells or Cell Lines

Cell Name Cell Type Source
K562 Cell line
Mouse neuronal cells Primary
Human haematopoetic stem cells Primary
Mouse embryonic stem cells Primary

Performance

Generation of longer length cDNA compared to convential SMARTer chemistry.

Publications or Articles

Not available

Performance data

Not available

Additional documents

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