Cells are loaded, stimulated with LPS, and incubated in-chip in a tissue culture incubator. After incubation, the cells are washed, lysed, reverse transcribed and amplified using Clontech SMARTer Ultra Low RNA Kit. This script has been updated to be compatible with all versions of the C1 Open App IFCs, including the redesigned medium 96 cell IFC.Please use 10,000 characters max
|Cell Name||Cell Type||Source|
|Bone marrow derived dendritic cells||Dendritic cells||Primary|
Stimulated 1,775 single dendritic cells, at various time courses for three pathogenic components (LPS, PIC (viral-like double-stranded RNA), and PAM (synthetic mimic of bacterial lipopeptides)) and additional perturbations. For most conditions, we captured up to 96 cells, and generated a matching population control. We sequenced each sample to an average depth of 4.5 +/- 3.0 million read pairs, as single-cell expression estimates stabilized at low read-depths.
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