C1 CAGE is a method for single-cell transcriptome analysis for molecular counting of RNA 5'-ends. Paired-end sequencing, random priming and unique molecular identifiers are used for single-molecule fragment assembly of mRNAs and long non-coding RNAs, including non-polyadenylated transcripts. This script has been updated to be compatible with all versions of the C1 Open App IFCs, including the redesigned medium 96 cell IFC.Please use 10,000 characters max
|Cell Name||Cell Type||Source|
|HeLa||Human cervix adenocarcinoma||Cell line|
|A549||Human lung carcinoma||Cell line|
C1 CAGE uses random priming, unique moloecular identifier (UMI) and paired-end sequencing to allow molecular counting of mRNAs and lomg non-coding RNAs, including non-polyadenylated transcripts. In this method, 66.2% reads represent 5' ends of RNA in average. Amongst the 5' end reads, approximately 10% of rRNA, 15% of spike reads and 50% of genome mapped reads are represented. The UMI counts the number of RNA molecules per single cell. In average 8,000 molecules per single cell were detected by Miseq sequencer.
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