C1 mRNA Seq HT maximizes your single-cell study size while minimizing library preparation and sequencing costs. This efficient workflow makes it easy for C1 users to pursue large-scale studies to explore heterogeneity and discover novel cell populations. The C1 mRNA Seq HT IFCs innovative architecture allows users to capture up to 800 single-cells and apply a cell barcode at first strand synthesis. Then, the IFC harvests barcoded cDNA into 20 multiplexed pools of 40 single cells each for library prep. This unique chemistryreduces both hands-on time and library preparation expense. The chemistry also employs a 3 end-counting strategy to increase sample pooling and throughput. Download the C1 mRNA Sequencing Data Sheet to learn more.Please use 10,000 characters max
|Cell Name||Cell Type||Source|
|K562||Human leukemia||Cell line|
|3T3||Mouse fibroblast||Cell line|
|Cortical Neurons||Human neuron||Primary|
|ESC||Mouse stem cell||Primary|
This protocol was tested on a diverse set of cell line and primary samples. We found that it routinely detects >4,000 genes per cell with TPM>1. For more information on performance, please see the C1 mRNA Sequencing Data Sheet.
Drag additional files here to upload browse