This protocol enables the lysis and whole genome amplification of bacterial cells isolated from standard lab conditions as well as from environmental samples. This protocol can be used to generate amplified genomic DNA from small groups of 5-10 cells, producing mini-metagenomes. The cell wash step is removed from the standard cell capture protocol. Instead, well suspended bacterial cells are loaded into the C1 IFC chambers via random Poisson distribution. Lysis is performed using Epicenter's Ready-Lyse, and MDA based amplification is performed using the Qiagen Repli-g sc kit.Please use 10,000 characters max
|Cell Name||Cell Type||Source|
|Microbial Sample||Environmental Microbial Communities|
During mini-metagenomic applications, 5-10 cells are randomly loaded into each chamber or capture site. This protocol performs MDA for 5 hours and can generate 10-200 ng of DNA at the output in 3.5 uL harvest reagent.
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