We developed an easy to use and efficient single cell total RNA sequencing workflow based on the SMARTer stranded total RNA-seq kit - pico input mammalian (Takara) including a ribodepletion step at the cDNA level with specific probes. Full length cDNA of polyadenylated as well as non-polyadenylated transcripts is generated using random primers and the SMARTer principle.Please use 10,000 characters max
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The method generates cDNA of polyadenylated as well as non polyadenylated transcripts.
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