SMARTer total RNA-seq

mRNA Sequencing

Verboom K. et al.
Center for Medical Genetics Ghent, Ghent University

(...) Published on Rev A

  • supported ifcs:
    Open App IFC
  • number of ifc runs:


We developed an easy to use and efficient single cell total RNA sequencing workflow based on the SMARTer stranded total RNA-seq kit - pico input mammalian (Takara) including a ribodepletion step at the cDNA level with specific probes. Full length cDNA of polyadenylated as well as non-polyadenylated transcripts is generated using random primers and the SMARTer principle.

Please use 10,000 characters max

Protocol: SMARTer single cell total RNA sequencingDuration (H:M): 8:00

Cell Load and Stain

Sample Prep

view protocol worksheet

Tested Primary Cells or Cell Lines

Cell Name Cell Type Source
NGP Neuroblastoma Cell line
SK-N-BE-2C neuroblasoma Cell line
SHSY5Y-MYCN-TR Neuroblastoma Cell line


The method generates cDNA of polyadenylated as well as non polyadenylated transcripts.

Publications or Articles

Performance data

Not available

Additional documents

Drag additional files here to upload browse