Overview

C1-RamDA-seq is a full-length total RNA-sequencing method for understanding single-cell localites such as non-poly(A) RNAs, pre-mRNA, and eRNA. This application will be valuable to researchers working rare cells such as in cancer biology, neurobiology and immune biology. As many biologically important cell types are rare and are often found in heterogeneous cell populations, the accumulating evidence suggests that there is an importance of gaining information at the full-length total RNA-seq level in single cells.

Protocol: C1-RamDA-seqDuration (H:M): 4:00

Tested Primary Cells or Cell Lines

Performance

C1 RamDA-seq profiles detects 15,000 transcripts with recursive splicing in >300-kb introns and detects enhancer RNAs and their cell type-specific activity in mouse embryonic stem cells. C1-RamDA-seq demonstrate the dynamics of gene expression, RNA-processing events and transcriptional regulation in single-cells.

Publications or Articles

• Single-cell full-length total RNA sequencing uncovers dynamics of recursive splicing and enhancer RNAs

Performance data

• Custom analysis codes

Additional documents