Publications

A ddRAD-based genetic map and its integration with the genome assembly of Japanese eel (Anguilla japonica) provides insights into genome evolution after the teleost-specific genome duplication

Kai, W., Nomura, K., Fujiwara, A., Nakamura, Y., Yasuike, M., Ojima, N., Masaoka, T., Ozaki, A., Kazeto, Y., Gen, K., Nagao, J., Tanaka, H., Kobayashi, T., Ototake, M.

Recent advancements in next-generation sequencing technology have enabled cost-effective sequencing of whole or partial genomes, permitting the discovery and characterization of molecular polymorphisms. Double-digest restriction-site associated DNA sequencing (ddRAD-seq) is a powerful and inexpensive approach to developing numerous single nucleotide polymorphism (SNP) markers and constructing a high-density genetic map. To enrich genomic resources for Japanese eel (Anguilla japonica), we constructed a ddRAD-based genetic map using an Ion Torrent Personal Genome Machine and anchored scaffolds of the current genome assembly to 19 linkage groups of the Japanese eel. Furthermore, we compared the Japanese eel genome with genomes of model fishes to infer the history of genome evolution after the teleost-specific genome duplication.

Citation

Kai, W., Nomura, K., Fujiwara, A., Nakamura, Y., Yasuike, M., Ojima, N., Masaoka, T., Ozaki, A., Kazeto, Y., Gen, K., Nagao, J., Tanaka, H., Kobayashi, T., Ototake, M. "A ddRAD-based genetic map and its integration with the genome assembly of Japanese eel (Anguilla japonica) provides insights into genome evolution after the teleost-specific genome duplication" BMC Genomics (2014): 233