Acquisition, processing, and quality control of mass cytometry data
Lee, B.H. and Rahman, A.H.Mass cytometry uniquely combines the principles of mass spectrometry and flow cytometry for high dimensional profiling of immune cells at a single cell level. Using isotopically conjugated antibodies, mass cytometry overcomes the limitations of spectral overlap associated with flow cytometry and allows for deeper single cell characterization of complex biospecimens using more cellular markers. However, the nature of mass spectrometry-based single cell measurements requires specific considerations in acquiring and processing data. This chapter provides an overview of how to optimally acquire mass cytometry data and how to process this data for subsequent analysis and characterization of cell populations.
Lee, B.H. and Rahman, A.H. "Acquisition, processing, and quality control of mass cytometry data" Mass Cytometry: Methods and Protocols (2019): 13–31