Another step on the path to mass cytometry standardization
For over 20 years, flow cytometry has been used to analyze samples on a single-cell basis. While six-or eight-color assays are sufficient for many routine tasks, more recent work has led to the improvement of both the hardware and reagents used in flow cytometry. The development of four-laser flow cytometers and probes with narrower, less-overlapping spectra has allowed up to at least 17-color experiments.
Leopold, M.D. "Another step on the path to mass cytometry standardization" Cytometry Part A (2015): 380–2