Cycling into Future: Mass Cytometry for the Cell-Cycle Analysis

Darzynkiewicz, Z.

Progress in the development of instrumentation and new methodologies is a driving force for advancement in biology and medicine. By offering a possibility to assess cell heterogeneity and identify individual cells or cell subpopulation having different properties, combined with multiparametric analysis, the cytometric methodologies are of particular value. Compared with the early means to interrogate individual cells by microspectrophotometry and autoradiography developed in 50s and 60s of the past century, the introduction and advances of flow cytometry during the last four decades revolutionized the way of cell analysis and contributed to the immense growth of our knowledge in many disciplines of cell and molecular biology. It had also found important clinical applications advancing diagnosis and treatment of many diseases (1). Analytical capabilities of exploring individual cells were further enriched by the development of imaging cytometry instrumentation, such as provided by laser scanning cytometry and imaging flow cytometry. Progressive improvements in flow and imaging cytometry led to unprecedented expansion of our knowledge of regulatory mechanisms in normal and diseased cells. Particularly, fruitful were applications of these methodologies in revealing mechanisms of cell proliferation as related to a multitude of molecules, signaling pathways and events controlling the cell-cycle progression.


Darzynkiewicz, Z. "Cycling into Future: Mass Cytometry for the Cell-Cycle Analysis" Cytometry, Part A (2012): 546–8