Publications

Maternal obesity is associated with phenotypic alterations in fetal immune cells by single‐cell mass cytometry

Enninga, E.A.L., Jang, J. S., Hur, B.

Problem

Prenatal exposure to metabolic dysregulation arising from maternal obesity can have negative health consequences in post‐natal life. To date, the specific effects of maternal obesity on fetal immunity at a cellular level have not been well characterized.

Method of Study

Using cord blood mononuclear cells (CBMCs) and cord plasma (n = 9/group) isolated from infants born to women with a high body mass index (BMI>25kg/m2) compared to women with a normal BMI (18‐25kg/m2), we evaluated differences in immune cell populations using single‐cell mass cytometry (CyTOF). CBMCs were matched according to potentially confounding variables, such as maternal and gestational age, ethnicity, smoking status, and gravidity. Statistical results were adjusted for fetal sex. Data were analyzed by viSNE and FlowSOM softwares in Cytobank.

Citation

Enninga, E.A.L., Jang, J. S., Hur, B. "Maternal obesity is associated with phenotypic alterations in fetal immune cells by single‐cell mass cytometry" American Journal of Reproductive Immunology (2020): doi.org/10.1111/aji.13358