Publications

Quantifying RNA allelic ratios by microfluidic multiplex PCR and sequencing

Zhang, R., Li, X., Ramaswami, G. et al.

We developed a targeted RNA sequencing method that couples microfluidics-based multiplex PCR and deep sequencing (mmPCR-seq) to uniformly and simultaneously amplify up to 960 loci in 48 samples independently of their gene expression levels, and accurately and cost-effectively measure allelic ratios even for low-quantity or low-quality RNA samples. We applied mmPCR-seq to RNA editing and allele-specific expression studies. mmPCR-seq complements RNA-seq and provides a highly desirable solution for future applications.

Citation

Zhang, R., Li, X., Ramaswami, G. et al. "Quantifying RNA allelic ratios by microfluidic multiplex PCR and sequencing" Nature Methods (2014): 51–4