Publications

Recurrent mTORC1-activating RRAGC mutations in follicular lymphoma

Jessica Okosun, J., Wolfson, R.L., Wang, J., Araf, S., Wilkins, L., Castellano, B.M., Escudero-Ibarz, L., Al Seraihi, A.F., Richter, J., Bernhart, S.H., Efeyan, A., Iqbal, S., Matthews, J., Clear, A., Guerra-Assunção, J.A., Bödör, C., Quentmeier, H., Mansbridge, C., Johnson, P., Davies, A., Strefford, J.C., Packham, G., Barrans, S., Jack, A., Du, M.Q., Calaminici, M., Lister, T.A., Auer, R., Montoto, S., Gribben, J.G., Siebert, R., Chelala, C., Zoncu, R., Sabatini, D.M., Fitzgibbon, J.

Follicular lymphoma is an incurable B-cell malignancy characterized by the t(14;18) and mutations in one or more components of the epigenome. Whilst frequent gene mutations in signaling pathways, including JAK-STAT, NOTCH and NF-κB, have also been defined, the spectrum of these mutations typically overlap with the closely-related diffuse large B cell lymphoma (DLBCL). A combination of discovery exome and extended targeted sequencing revealed recurrent somatic mutations in RRAGC uniquely enriched in FL patients (17%). More than half of the mutations preferentially co-occurred with ATP6V1B2 and ATP6AP1 mutations, components of the vacuolar H+-adenosine triphosphate ATPase (v-ATPase) known to be necessary for amino acid-induced mTORC1 activation. The RagC mutants increased raptor binding whilst rendering mTORC1 signaling resistant to amino acid deprivation. Collectively, the activating nature of the RRAGC mutations, their existence within the dominant clone and stability during disease progression supports their potential as an excellent candidate to be therapeutically exploited.

Citation

Jessica Okosun, J., Wolfson, R.L., Wang, J., Araf, S., Wilkins, L., Castellano, B.M., Escudero-Ibarz, L., Al Seraihi, A.F., Richter, J., Bernhart, S.H., Efeyan, A., Iqbal, S., Matthews, J., Clear, A., Guerra-Assunção, J.A., Bödör, C., Quentmeier, H., Mansbridge, C., Johnson, P., Davies, A., Strefford, J.C., Packham, G., Barrans, S., Jack, A., Du, M.Q., Calaminici, M., Lister, T.A., Auer, R., Montoto, S., Gribben, J.G., Siebert, R., Chelala, C., Zoncu, R., Sabatini, D.M., Fitzgibbon, J. "Recurrent mTORC1-activating RRAGC mutations in follicular lymphoma" Nature Genetics (2016): 183–8