Single-Cell Protein Assays: A Review

Fan, B., Wang, J., Xu, Y. et al.

Quantification of single-cell proteomics provides key insights in the field of cellular heterogeneity. This chapter discusses the emerging techniques that are being used to measure the protein copy numbers at the single-cell level, which includes flow cytometry, mass cytometry, droplet cytometry, microengraving, and single-cell barcoding microchip. The advantages and limitations of each technique are compared, and future research opportunities are highlighted.


Fan, B., Wang, J., Xu, Y. et al. "Single-Cell Protein Assays: A Review" Methods in Molecular Biology (2018): 293-309