CYTO 2019

June 22–26, 2019 | Vancouver Convention Center, Vancouver, Canada


Introducing the new standard in immune profiling

Highly multiplexed mass cytometry and Imaging Mass Cytometry™ are helping to transform our understanding of the immunome by delivering fresh insights into cellular phenotype and function and the tissue microenvironment.

Innovative products like the recently introduced Maxpar® Direct Immune™ Profiling System for the Helios™ mass cytometer are setting new standards in immunophenotyping, while the Hyperion™ Imaging System provides deeper contextual insight into immune infiltration of tissues and tumors.

Visit us at Booth 426 to learn more.

Download the CYTO 2019 Fluidigm Presence Flyer for a list of presentations and posters on mass cytometry at CYTO 2019.

Join us at our CYTO 2019 commercial tutorial

Deep profiling of immune cell phenotypes and function from circulation to tissue with CyTOF® technology

Sunday, June 23 | 12:30–1:30 pm | Room 1

BOX LUNCH IS PROVIDED. SEATING IS LIMITED.

Speakers

Michelle Poulin, PhD

Michelle Poulin, PhD
Manager, Field Applications
Fluidigm

Thomas Ashhurst, BSc

Thomas Ashhurst, BSc
High-Dimensional Cytometry Specialist, Sydney Cytometry Facility
University of Sydney

Highly multiplexed mass cytometry and Imaging Mass Cytometry (IMC™) are transforming our understanding of the immunome. Michelle Poulin, PhD, will present “The new standard for deep immune profiling with CyTOF technology: 30 markers, 1 tube, 5-minute data analysis.” She will describe the Maxpar Direct Immune Profiling System, an exciting new product providing a sample-to-answer solution that enables enumeration of 37 immune cell populations from human PBMC and whole blood. Data on repeatability, software precision and accuracy and site-to-site reproducibility will be shown.

Then, Thomas Ashhurst, BSc, will present “Investigating immune infiltration during viral encephalitis using Imaging Mass Cytometry,” highlighting his research on how viral infection of the central nervous system stimulates a rapid influx of bone marrow-derived monocytes and macrophages into the parenchyma. He will describe use of IMC to perform high-dimensional tissue profiling to gain a more comprehensive analysis of the key factors involved in this mobilization of the immune system.

View product presentations

The need to distinguish an ever-increasing array of newly identified cell types in size-limited samples is driving interest in higher-parameter cytometry. Mass cytometry and Imaging Mass Cytometry provide much-needed solutions, and both are now offered as a service in core facilities around the world.

Visit us at Booth 426 to learn more.

Exhibit dates and hours

Sunday, June 23 6:00–8:00 pm
Monday, June 24 10:00 am–6:00 pm
Tuesday, June 25 10:00 am–6:00 pm
Wednesday, June 26 10:00 am–4:00 pm

Featured Products

Come visit our booth and learn how you can deeply profile tumors and the tumor immune response from every angle.

Maxpar® Direct™ Immune Profiling Assay™

The first complete sample-to-answer solution for high-dimensional immune profiling of human PBMC and whole blood. The system brings together CyTOF technology, a dry 30-marker antibody panel and automated analysis with Maxpar Pathsetter™ software to easily quantify 37 immune cell populations in a single tube.

Maxpar Direct Immune Profiling Assay


Helios, a CyTOF System

Identify known and novel immune cell signatures using a wide range of cell surface and intracellular proteomic biomarkers.

Helios


Hyperion Imaging System

The Hyperion Imaging System enables you to comprehensively interrogate complex cellular phenotypes and their interrelationships in the context of the tumor microenvironment with Imaging Mass Cytometry.

Hyperion System


Visit posters and presentations featuring Fluidigm technology

CYTO® 2019 features many talks and posters focusing on mass cytometry and Imaging Mass Cytometry as key components in the discovery of new cell types and fresh insights into cellular function and the tissue microenvironment.

Attend the presentations listed below and visit us at Booth 426 for a complete list of talks and posters.

Oral presentations | Saturday, June 22–Wednesday, June 26

Saturday, June 22

Time

Title

Presenter and Institution

Session

8:30–10:00 am Data-Mining Techniques for Single-Cell Data Yvan Saeys
VIB-Ghent University, Belgium
Scientific Tutorial 4
10:30 am–12:00 pm Best t-SNE on the Block: How to Achieve a Meaningful Low-Dimensional Embedding and Interpret It Correctly Anna Belkina
Boston University

Josef Spidlen
FlowJo
Scientific Tutorial 8
1:00–2:30 pm Advanced Data Analysis in a Shared Resource Laboratory Sofie Van Gassen
VIB-UGent Center for Inflammation Research, Ghent University

Dagna Sheerar, SCYM(ASCP)
University of Wisconsin Comprehensive Cancer Center Flow Cytometry Laboratory
Scientific Tutorial 15

Sunday, June 23

Time

Title

Presenter and Institution

Session

8:30–10:00 am Translating a Trillion Points of Data into Therapies, Diagnostics, and New Insights into Immunology Atul Butte
University of California, San Francisco
State of the Art Lectures: Single Cell Flow and Beyond
10:30 am–12:00 pm Multiview Machine Learning Increases Predictive Power in Clinical Flow and Mass Cytometry Datasets Natalie Stanley
Stanford University
Parallel Session 2: New Algorithms I
10:30 am–12:00 pm A Computational Approach for Automated Biomarker Discovery: Identification of Cell Subpopulations Involved in Acute Response to Chikungunya Virus with CITRUS in Cytobank Geoff Kraker
Cytobank
Parallel Session 2: New Algorithms I
1:45–3:15 pm "Google Maps" for Tissue Biology–How to Find Topographic Biomarkers Denis Schapiro
Harvard Medical School and Broad Institute
Plenary Session 1: 3D Cytometry
3:30–4:30 pm How to Ensure Robustness and Reproducibility when Using Mass Cytometry for Clinical Studies Facilitators:
Jose Estevam, Diana L. Bonilla Escobar, Holden Maecker, Adeeb Rahman, Jonathan Irish, James Lederer, Brice Gaudillière, Nima Aghaeepour, Elena Hsieh, Greg Behbehani, Mike Leipold, Radhika Rayanki, Peter Krutzik, Chris Grove
Concurrent Workshop 5

Monday, June 24

Time

Title

Presenter and Institution

Session

10:30 am–12:00 pm High Risk Glioblastoma Cells Revealed by Machine Learning and Single Cell Signaling Profiles Jonathan Irish
Vanderbilt University
Parallel Session 5: Application of Multimodal Analysis to Disease Biology

Tuesday, June 25

Time

Title

Presenter and Institution

Session

10:30 am–12:00 pm Exploring Immune Cell Dynamics in Inflammatory Bowel Disease and Its Therapeutic Modulation by TNF-alpha Blockade Sabine Baumgart
German Rheumatism Research Center (DRFZ)
Parallel Session 10: Application of High Parameter Cytometry to Disease II
10:30 am–12:00 pm Machine Learning for Integration of Mass Cytometry with the Transcriptome, Microbiome, Proteome, and Metabolome for Prediction of Clinical Outcomes Mohammad Sajjad Ghaemi
Stanford University
Parallel Session 12: New Algorithms II
10:30 am–12:00 pm Cell-Type Specific Subspace Clustering Analysis of High-Dimensional Flow and Mass Cytometry Data for Data-Driven Identification of Difficult-to-Resolve Cell Populations Yu Qian
J. Craig Venter Institute
Parallel Session 12: New Algorithms II
10:30 am–12:00 pm Deep Profiling of the Neonatal Immune System Using CyTOF Laura Peterson
Stanford University
Parallel Session 13: Immune Landscapes
10:30 am–12:00 pm From Bone Marrow to Peripheral Blood: Incidence of the G-CSF-Mediated Mobilization on Cellular Landscape Lea Guyonnet
Luxembourg Institute of Health
Parallel Session 13: Immune Landscapes
10:30 am–12:00 pm A Modified Injector and Sample Acquisition Protocol Improves the Data Quality and Reduces the Inter-Instrument Variability of the Helios Mass Cytometer Brian Lee
Icahn School of Medicine at Mount Sinai
Parallel Session 14: Standardization, Performance, Optimization

Wednesday, June 26

Time

Title

Presenter and Institution

Session

10:30 am–12:00 pm Experimental Severe Malaria Is Resolved by Targeting Newly-Identified Monocyte Subsets with Immune Modulatory Treatment Paula Niewold
University of Sydney
Parallel Session 15: Cytometry in Infectious Disease and Asthma
10:30 am–12:00 pm Identification of a Sepsis-Specific Neutrophil Cellular Signature Using Mass Cytometry Approach Aïda Meghraoui-Kheddar
Sorbonne University
Parallel Session 15: Cytometry in Infectious Disease and Asthma
10:30 am–12:00 pm Ditch the Ball: Automated Optimal Parameters for T-Distributed Stochastic Neighbor Embedding Improve Visualization and Allow Analysis of Large Datasets Anna Belkina
Boston University
Parallel Session 17: Application of Algorithms
10:30 am–12:00 pm High-Dimensional Single Cell Mass Cytometry Analysis of the Murine Hematopoietic System Reveals Distinct Alterations Induced during Immunoaging Versus Physiological Pathogen Challenges Christos Nikolaou
Berlin-Brandenburg Center for Regenerative Therapies
Parallel Session 18: Biological Insights I
1:45–3:15 pm Mass Cytometry Profiling of Cell Signaling Pathways in Active and JAK Inhibitor-Treated Systemic Lupus Erythematosus Patients Marie Urbicht
German Rheumatism Research Center (DRFZ)
Parallel Session 19: Mass Cytometry in Disease Studies
1:45–3:15 pm CyTOF plus DISCOV-R Analysis Link the Phenotype of Rare Autoreactive T Cells with Disease Outcome in Type 1 Diabetes Alice Wiedeman
Benaroya Research Institute at Virginia Mason
Parallel Session 19: Mass Cytometry in Disease Studies
1:45–3:15 pm Glucocorticoid-Resistant Leukemic B-cells Undergo a Phenotypic Change that Increases Sensitivity to SRC/ABL Inhibition Jolanda Sarno
Stanford University
Parallel Session 19: Mass Cytometry in Disease Studies
1:45–3:15 pm A Year-Long Immune Profile of the Systemic Response in Acute Stroke Survivors Amy Tsai
Stanford University
Parallel Session 19: Mass Cytometry in Disease Studies
1:45–3:15 pm Novel Chemical Platform for the Mass Cytometry-Based Multiparametric Analysis of Cancer Activome Katarzyna Groborz
Wroclaw University of Science and Technology
Parallel Session 22: Biological Insights II
4:30–5:15 pm Mass Cytometry Track Open discussion Meeting Track Wrap-Up

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