Is preamplification required in the Advanta IO Gene Expression Assay?

Yes, preamplification in the Advanta™ IO Gene Expression Assay is required to achieve the performance characteristics of the assay. The protocol includes instructions for performing preamplification, and each kit includes the required reagents and pooled primers. The protocol also provides instructions for adding up to 17 custom TaqMan® Assays (each at 20X concentration) to the preamplification pool for Panel B.

For Research Use Only. Not for use in diagnostic procedures.