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How much tissue should I start with?

The Advanta™ FFPE RNA Extraction Kit Protocol (PN 101-6554) is applicable for extraction of RNA from FFPE tissue sections that are 5–10 µm thick with an area of 20–200 mm2, for a total volume of up to 2.0 mm3 of tissue in each reaction. Due to variations in the composition of different tissues and sample types, we recommend that you test input amounts for each tissue type. Too much tissue in the extraction reaction adversely affects the RNA yield.


For Research Use Only. Not for use in diagnostic procedures.