Can I prepare a master mix for steps that require mixing multiple reagents?

A few steps that direct the user to pipet reagents individually into each sample tube can be modified into a master mix preparation for easier pipetting when more than a few sample tubes are processed at a time. Prepare about 25% volume overage for master mixes. A master mix could be prepared for:

•    Protease K digestion mixture

•    Bead cleanup mixture

It is not recommended to prepare a master mix for the DNase I digestion step because adding the DNase I enzyme into the 10X DNase reaction buffer directly could impact enzyme performance.

For Research Use Only. Not for use in diagnostic procedures.