How do you prepare and stain your cells?
Sample prep is very similar to what is done for traditional flow cytometry, meaning that cell suspensions are stained in tubes with a panel of tagged antibodies directed against an array of cellular protein targets. It is important to use metal-free sample processing buffers (see our catalog). The final staining step includes a nucleic acid intercalator that is used to identify nucleated events.
We have validated protocols for staining all three cellular compartments (surface, cytoplasm and nucleus).