How do you prepare and stain your cells?

Sample prep is very similar to what is done for traditional flow cytometry, meaning that cell suspensions are stained in tubes with a panel of tagged antibodies directed against an array of cellular protein targets. It is important to use metal-free sample processing buffers (see our catalog). The final staining step includes a nucleic acid intercalator that is used to identify nucleated events.

We have validated protocols for staining all three cellular compartments (surface, cytoplasm and nucleus).

For Research Use Only. Not for use in diagnostic procedures.