What is the maximum number of preamplification cycles I can do?
No maximum number of preamplification cycles has been established. However, if there are major efficiency differences between the primer sets, increasing the number of cycles will exacerbate those differences in the preamplified material. Fluidigm recommends 18 preamplification cycles when using starting material as low as 10 pg of total RNA.
For Research Use Only. Not for use in diagnostic procedures.