An Integrated RNA-Seq Workflow for Significant Cost Savings on Reagents and Labor

Presenter: Steve Kain, Microfluidics Marketing

Abstract: RNA sequencing (RNA-seq) has become the standard for expression profiling methods in cells, tissues and organisms. We have developed an elegant microfluidics-based chemistry and workflow called the Advanta™ RNA-Seq XT NGS Library Prep Kit. Leveraging the Juno™ instrument, our RNA-seq workflow supports simultaneous processing of up to 48 samples with a one-click script on our instrument. We have recently updated our reagent kits to include unique dual index (UDI) adapters, ideal for use on Illumina® NovaSeq™ platforms, as well as simplifying the workflow for additional savings on hands-on time and labor costs. The Advanta RNA-Seq XT Kits include reagents necessary to generate random-primed, stranded RNA-seq libraries from the polyadenylated RNA fraction present in as little as 10 ng of total RNA from eukaryotic organisms.