C1
Characterize selected cell populations with high molecular detection sensitivity using a broad menu of multi-omic single-cell assays.
The world’s first automated solution for single-cell genomics research continues to break new ground, now offering more capabilities than ever before. C1 lets you prepare single-cell templates for mRNA sequencing, DNA sequencing, epigenetics or miRNA expression.
Technology
The innovative C1 system carefully isolates single cells into individual reaction chambers in the exclusive Fluidigm integrated fluidic circuit (IFC). The optically clear IFC lets you automatically stain captured cells and examine them by microscopy for viability, surface markers or reporter genes. After staining, cells are automatically lysed and template is quickly prepared for qPCR or sequencing analysis—all in as little as four hours.
Workflow
1
Prime
Prime the C1 IFC in preparation for sample transfer.
2
Capture
Insert the IFC into C1 to automatically isolate up to 800 individual cells to separate reaction chambers.
3
Stain
Label each cell and microscopically image the IFC to confirm cell count, viability or phenotype.
4
Lyse
Reinsert the IFC into C1 to lyse each cell for amplification.
5
Amplify
Magnify each cell’s RNA or DNA content for sequencing or qPCR detection.
6
Harvest
Collect product from the C1 outlet.
Publications
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C1 mRNA Seq HT Demultiplex Script
The C1™ mRNA Sequencing High Throughput Demultiplexer Perl script application programming interface (API) allows automatic demultiplexing of individual single-cell samples from each column using th...
C1 System Software
The C1™ system supports DNA sequencing, mRNA sequencing, targeted gene expression, miRNA analysis and additional applications through the C1 Open App™ IFC (integrated fluidic circuit). Download the...
Script Builder Software
C1™ Script Builder™ allows you to create innovative single-cell applications to deeply explore cellular heterogeneity and the functional attributes of every cell population. Download the latest ver...
Singular Analysis Toolset Software
An innovative, open-source solution for analyzing and visualizing data, the Singular™ Analysis Toolset offers powerful methods for identifying gene expression and mutation patterns at the single-ce...
Support
A critical success factor when adopting high-performance technology is acquiring the proficiency and support necessary to accelerate your research and discovery initiatives. Fluidigm PRO Services are a comprehensive portfolio of solutions to help you maximize your investment, scale up productivity and empower your laboratory with greater access to professional resources.
C1™ Single-Cell Reagent Kit for Preamp
Sufficient reagents required for cell capture, wash and harvest for 10 C1 IFCs for Preamp (960 samples).Kit contains:Module 12 bottles of C1 Cell Wash Buffer, 25.9 mL10 tubes of C1 Suspension Reagent, 50 µL2 tubes of C1 Blocking Reagent, 180 µLModule 210 tubes of C1 Harvest Reagent, 2.2 mL2 tubes of C1 Preloading Reagent, 120 µL2 tubes of C1 Loading Reagent, 25 µL2 tubes of C1 Lysis Plus Reagent, 30 µL2 tubes of C1 DNA Dilution Reagent, 17.7 mL2 tubes of C1 Preamp Dilution Reagent, 250 µLIFCs sold separately. Product insert available.
C1™ Single-Cell Reagent Kit for mRNA Seq
Sufficient reagents required for cell capture, wash and harvest for 10 C1 IFCs for mRNA Seq (960 samples).Kit contains:Module 12 bottles of Cell Wash Buffer, 25.9 mL10 tubes of Suspension Reagent, 50 µL2 tubes of C1 Blocking Reagent, 180 µLModule 22 bottles of C1 DNA Dilution Reagent, 17.7 mL2 tubes of Loading Reagent, 1.0 mL4 tubes of C1 Preloading Reagent, 120 µL11 bottles of C1 Harvest Reagent, 3.7 mLIFCs sold separately. Product insert available.
C1™ Single-Cell mRNA Seq HT Reagent Kit v2 - 5 IFC
Sufficient barcodes, primers and reagents required for cell capture, 3' end counting and cell multiplexing for 5 C1 Single-Cell mRNA Seq HT IFCs (4,000 samples).IFCs sold separately. Replaces product 101-0063.Kit contains:C1 Single-Cell mRNA Seq Reagent Kit, Module 15 tubes of 10X Blocking Reagent, 50.0 µL7 tubes of Cell Rinsing Reagent, 1.6 mL1 tube of Stability Solution, 1.0 mL5 tubes of Suspension Reagent, 50.0 µL3 tubes of Valve Fluid V2, 1.5 mL2 tubes of Actuation Fluid, 1.5 mLC1 Single-Cell mRNA Seq Reagent Kit, Module 25 bottles of C1 Harvest Reagent, 3.7 mL1 tube of DNA Dilution Reagent, 1.0 mL1 tube of Enrichment Primer, 130.0 µL5 tubes of Loading Reagent, 25.0 µL1 tube of Preamp Primer, 15.0 µL5 tubes of Preloading Reagent, 750.0 µLC1 Single-Cell mRNA Seq Reagent Kit, Module 35 Cell Barcode Plates40 barcodesC1 Single-Cell mRNA Seq Reagent Kit, Module 41 tube of Reverse Transcription Primer, 20.0 µLC1 Single-Cell mRNA Seq Reagent Kit, Module 51 tube of 5X First-Strand Buffer, 96.0 µL1 tube of dNTP Mix (20 mM each), 30.0 µL1 tube of Dithiothreitol (DTT; 100 mM), 15.0 µL1 tube of Reverse Transcriptase (100U/µL), 48.0 µL1 tube of Nuclease-Free Water, 1.0 mL1 tube of RNase Inhibitor (40U/µL), 60.0 µL1 tube of 10X Lysis Buffer — v3, 260.0 µLIFCs sold separately. Product insert available. Replaces product 101-0063 C1™ Single-Cell mRNA Seq HT Reagent Kit.
C1™ Single-Cell Preamp IFC, 5–10 µm—10 IFCs
Ten C1 IFCs for Preamp, capture, lysis, reverse transcription and preamplification of 96 individual small cells (5–10 µm). Kit contains: 10 IFCs.Reagents sold separately.
C1™ Single-Cell Preamp IFC, 17–25 µm—10 IFCs
Ten C1 IFCs for Preamp, capture, lysis, reverse transcription and preamplification of 96 individual large cells (17–25 µm).Kit contains: 10 IFCsReagents sold separately.
C1™ Single-Cell mRNA Seq IFC, 10–17 µm—10 IFCs
Ten C1 IFCs. Each IFC is for mRNA Seq for capture, lysis, reverse transcription and cDNA amplification of 96 individual medium cells (10–17 µm).Kit contains: 10 IFCsReagents sold separately.
Resources
Flyers & brochures
Technical documents
Product inserts
Unless explicitly and expressly stated otherwise, all Fluidigm products are provided for Research Use Only, not for use in diagnostic procedures. For more information see www.fluidigm.com/legal/notices