CEL-Seq 2
mRNA sequencing

Hashimshony, T. and Yanai, I.
Yanai: Technion, Israel Institute of Technology

View protocol worksheet

Download script

 

PUBLISHED ON: SEPTEMBER 13, 2016 | REV A
SUPPORTED IFCS: Open App IFC
NUMBER OF IFC RUNS: 6

 

Overview

CEL-Seq2 is a 3'-end counting mRNAseq method that uses in vitro transcription for initial amplification. CEL-Seq2 incorporates numerous improvements over the original method including increased sensitivity and incorporation of unique molecular identifiers (UMIs). This script has been updated to be compatible with all versions of the C1™ Open App™ IFCs, including the redesigned medium 96-cell IFC.

Protocol: CEL-seq2  | Duration (H:M): 18:00

Tested Primary Cells or Cell Lines

Cell Name

Cell Type

Source

primary mouse fibroblasts Primary

Performance

Publication documents increased number of genes detected per cell compared to manual method


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