No more compromising for quality. With the Biomark™ HD system, you get reliable production-scale throughput and exquisite single-cell sensitivity, with a wide variety of sample types and chemistry choices to accommodate the most genomic applications. Now you can run entire projects from research to validation to routine screening on a single system with the flexibility you want and the consistency you need. Anything else we can help with?
Design experiments to match your needs across the widest range of genomic applications.
Detect unique molecular signatures in individual cells and rare cell populations.
Accelerate discovery with a higher throughput and automated workflow.
Conserve precious samples—and funding—with lower reagent use and nanoliter reactions.
Fluidigm’s revolutionary integrated fluidic circuits (IFCs) empower life science research by automating PCR reactions in nanoliter volumes. This means using less sample and reagent, and a single microfluidic device, to achieve the high-quality, consistent results your work depends on. The Biomark HD system runs IFCs in either real-time or end-point read modes, bringing flexible, efficient and economical PCR solutions to a range of applications.
Prime the Dynamic Array IFC to prepare it for sample transfer.
Pipet samples and primer-probe sets into the designated inlets on the IFC.
Load the IFC on the controller, which prepares the reactions.
Place the IFC into Biomark for thermal cycling and end-point or real-time fluorescence detection.
View and analyze color-coded expression heat maps, allele maps and cluster plots for single or multiple runs with our analysis software.
Biomark & EP1 Support
Explore tutorials and publications, browse frequently asked questions, download technical documents, review training programs and service plans and locate the right IFCs, kits and reagents for your research. Still need help? Reach out to one of our support specialists here.
IFCs, Assays and Reagents
Digital Array IFCs
Ensure a precise determination of copy number variation with this innovative IFC designed specifically for digital PCR experiments.
Dynamic Array IFCs
D3 Assay Design
SNP Type Assays
Delta Gene Assays
Realize the full potential of your Biomark™ HD system with minimal setup time and validated protocols that yield quality gene expression results. Just specify your genes of interest and target species to order our exclusive, high-quality, economical and MIQE-compliant Delta Gene™ assays (formerly DELTAgene). Designed specifically for use with Dynamic Array IFCs.
Biomark & EP1 Software
The Biomark/EP1 software package includes:
Data Collection Software v4.2.2
SNP Genotyping Analysis v4.3.2
SNP Genotyping software analyzes genotyping data acquired from Biomark™ or EP1 systems. Advanced features include a cluster-calling algorithm, multiple IFC scoring and automatic confidence score ratings.
Real-Time PCR Analysis v4.3.1
Real-Time PCR Analysis software helps you quickly and easily create standard curves, calculate DDCt and automatically determine the ideal threshold setting for each run. Specifically created for Biomark™ systems.
Digital PCR Analysis v4.1.2
Our innovative Digital PCR Analysis software offers real-time and endpoint dPCR analysis,
automated copy number variation, and the ability to quantitate up to four targets per sample.
Designed for use with Biomark™ and EP1™ systems.
Melt Curve Analysis v4.1.3
Instrument (Part Number BMKHD-BMKHD)
|Depth||75 cm (29.5 in)|
|Width||75 cm (29.5 in)|
|Height||100 cm (39.4 in)|
|Weight||140 kg (308 lb)|
|Thermal Control||Peltier-based, 4–99 ºC|
|Heating ramp rate||Up to 5.5 ºC/sec|
|Cooling ramp rate||Up to 5.5 ºC/sec|
|Detection||CCD camera; 9M pixel, 12 μm x 12 μm pixel size|
|Fluorescence excitation||475 nm, 530 nm, 575 nm|
|Fluorescence emission||525 nm, 570 nm, 630 nm|
|Power requirements||100–230 VAC, 50/60 Hz, 300 W
Fluidigm provides a region-specific power cord for the Biomark HD system.
|Instrument Control Computer|
|Computation||Intel® Core™ i3-2120, 3.30 GHz|
|Storage||500 GB HDD|
|Operating System||Microsoft® Windows® Professional Embedded 7|
|Monitor||LCD flat panel|
|Accessories||Keyboard and mouse|
Work Environment (indoor use only)
|Temperature||15–30 ºC (59–86 ºF)|
|Humidity||20%–80% relative humidity, non-condensing|
|Altitude||Not to exceed 2,000 m (6,560 ft) above sea level|
|Gene expression||48.48 Dynamic Array™ IFC
96.96 Dynamic Array IFC
192.24 Gene Expression IFC
Flex Six™ Gene Expression IFC
|Genotyping||48.48 Dynamic Array IFC
96.96 Dynamic Array IFC
192.24 Genotyping IFC
Flex Six Genotyping IFC
|Digital PCR||qdPCR 37K™ IFC
48.770 Digital Array™ IFC
12.765 Digital Array IFC
|Data Collection||Biomark HD Data Collection software|
|Analysis||Real-Time PCR Analysis software
SNP Genotyping Analysis software
Digital PCR Analysis software
Melt Curve Analysis software