Power to Proteomics
A breakthrough in cell analysis, the CyTOF® 2 Mass Cytometer brings the capability for simultaneous detection of many proteins to single-cell biology and boosts understanding of both cell type and function at the single-cell level. Using stable metal isotopes and time-of-flight mass cytometry, the CyTOF 2 provides more than 120 detection channels to dramatically advance your understanding of the fundamental unit of life.
Gain unprecedented insight into your biological system with over 100 detection channels
Profile each cell phenotypically and functionally, including distinction of normal and diseased states
Incorporate mass cytometry, Maxpar® reagents, assay design and cloud-based analysis
The world's only mass cytometry system, CyTOF 2 combines the advantages of high-speed single-cell analysis with the ability to resolve more than 120 metal probes, with minimal signal overlap. When used with Maxpar reagents, more than 36 proteins can be analyzed simultaneously in a single tube. It’s the opportunity to study the functional complexity of biological systems at the single-cell level, all with one instrument.
Stain cells according to targets of interest with metal-conjugated probes.
Inject prepared cells into CyTOF 2, which releases and ionizes the probes in individual cells, separates them by mass and counts the number bound to each cell.
View and analyze high-dimensional data sets through a convenient cloud-based platform, Fluidigm Cytobank.
IFCs, Assays and Reagents
Achieve reliable functional and phenotypic profiling of cell samples with antibodies sourced from the best vendors and validated for functionality and compatibility.
Analyze a variety of proteins in cell populations with our exclusive, ready-to-use kits containing up to 17 metal-conjugated antibodies.
Metal Labeling Kit
Conjugate metals to antibodies or intracellular proteins in as little as two and a half hours with our Maxpar labeling kits, optimized for use with CyTOF instruments.
Sample Prep Buffers
Optimize your mass cytometry staining with our sample prep buffers, which can be used in surface, intracellular and DNA staining protocols.
Cell-ID Cell Labeling Reagent
Determine live versus dead cells using the Cell-ID™ two cell-staining reagents, Intercalator-Ir and Intercalator-103Rh, to reliably characterize your sample.
Fluidigm Cytobank Analysis
Cloud-based data analysis platform provides complete control over your experiment and analysis approach, as well as the ability to share your data with colleagues and collaborators using histograms, bivariate plots, SPADE clustering and special heat maps optimized to facilitate analysis of high-dimensional CyTOF data sets. Created for use with CyTOF systems.
CyTOF 2 System
|Height||52 in (132 cm)|
|Width||38 in (97 cm)|
|Depth||31 in (79 cm)|
|Weight||628 lb (285 kg)|
|Mass range||89–209 amu|
|Abundance sensitivity||0.3% for 159Tb|
|Instrument response||400,000 counts/pg 159Tb|
|Detection limit||500 antibodies/cell|
|Dynamic range||4.5 orders of magnitude|
|Operating system||Windows® 7 Pro 64-bit|
|Data storage||720GB RAID (mirrored)|
|Connections||4 USB ports|
|Sample introduction||Dual loop, syringe|
|Peak throughput||1,000 (events/sec)|
|Flow rate||45 (µL/min)|
|Replicate sample CV (normalized)||<3%|
|Height||25 in (64 cm)|
|Width||15 in (38cm)|
|Depth||27 in (69 cm)|
|Weight||178 lb (81 kg)|
|Height||18 in (46 cm)|
|Width||8 in (20 cm)|
|Depth||23 in (58 cm)|
|Weight||77 lb (35 kg)|
|Operating system||Windows 7 Pro 64-bit|
|CPU||Intel® Core™ i7 3930K|
|RAM||8GB 2,400 MHz DDR3|
|Hard drives||1.44TB (6X 240GB SSD Corsair® Force Series™ GS SATA3) in RAID 10 config|
|Data storage||720GB RAID (mirrored)|
|Video||NVIDIA® NVS™ 310 x16 512MB|
|Power supply||650 W Antec TruePower|
|Serial adapter board||8 Port Native PCI Express RS232|
|Monitor||ViewSonic® 24 in LED|
|Keyboard/mouse||Logitech® MK120 Wired|
|Motherboard||ASUS® P9X79 WS PRO|
|Enclosure||Antec Titan 650|
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