The Future is Functional Genomics
Imagine if you could explore the genomes of every cell to find the gene networks that regulate them. You could correlate a cell’s phenotype, physiological condition and molecular signature to discover the genetic circuits regulating cell function or compare a population’s sensitivity to environmental changes. It’s the future of single-cell biology, and it’s available now on Polaris™, the first system to seamlessly integrate cell and molecular biology at the single-cell level. Select individual cells, challenge them with factors you choose under strict environmental conditions for up to 24 hours and measure each cell’s acute response transcriptionally via next-generation sequencing. And because it all happens on one precisely designed IFC, controlled by an intuitive touchscreen interface, all that’s left to the imagination is what question you’ll answer next.
Select your target cell by phenotype, viability or transgenic reporter.
Select, dose and prepare cells for analysis in one integrated workflow.
Observe and manipulate cells in their biological environment.
Challenge cells with a variety of parameters with the tap of a touchscreen.
An innovative integrated fluidic circuit (IFC) enables Polaris to actively select single cells from heterogeneous cell samples and hold them in individual, environmentally controlled reaction chambers. Once isolated, cells can be challenged with a variety of factors, including transcription factors, RNAs and small molecules, at the dosage or intervals you define. Then the cells are automatically lysed and RNA template is prepared for mRNA sequencing analysis. Polaris does it all in a single IFC and with minimal hands-on time, controlled by an elegant, intuitive touchscreen interface.
Prime the Polaris IFC with extracellular matrix and buffers to prepare it for sample transfer.
Pipet live cells in suspension and reagents into the IFC.
Insert the IFC into Polaris, which automatically images, selects and isolates individual cells according to your specifications.
Challenge selected cells with a variety of parameters and stain, if desired.
Lyse each cell to reverse transcribe and amplify within the IFC.
Collect the product from the harvest output for library prep and sequencing.
Browse frequently asked questions, download technical documents, review training programs and service plans and locate the right IFCs, kits and reagents for your research. Still need help? Reach out to one of our support specialists here.
IFCs, Assays and Reagents
Select, perturb and observe transcriptional changes in individual cells with the Polaris™ IFC. Our most sophisticated microfluidic IFC integrates cell biology and genomic analysis into one workflow so you can find the gene networks that regulate each cell. Designed for use with the Polaris system.
Isolate, stain, lyse and amplify single cells in one precise yet flexible workflow—all in as little as four hours with the innovative C1 system.
Dimensions as Shipped
|Height||42.0 in (106.8 cm)|
|Width||28.6 in (72.7 cm)|
|Depth||37.3 in (94.8 cm)|
|Weight||300 lb (136 kg)|
|Height||27.8 in (70.6 cm)|
|Width||20.6 in (52.2 cm)|
|Depth||34.1 in (86.6 cm)|
|Weight||242 lb (110 kg)|
|Clearance||6 in (15 cm) from the rear|
|Temperature||15–28 ºC (59–82 ºF) stable|
|Altitude site preparation||<8,202 ft (2,500 m)|
|Current||10 A (max)|
|Voltage||90 V (min) to 240 V (max)|
|Main supply fluctuation||not to exceed ±10% of the nominal supply voltage|
|Connection||4x USB 2.0|
|Grade||Clean, dry premixed gas (standard grade)|
|Blood gas||5% CO2, 5% oxygen and 90% nitrogen|
|Mixed gas||5% CO2, 20% oxygen and 75% nitrogen|