Explore tutorials and publications, browse frequently asked questions, download technical documents, review training programs and service plans and locate the right IFCs, kits and reagents for your research. Still need help? Reach out to one of our support specialists here.
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Publications
Successful targeted sequencing depends on quality library preparation, and successful prep depends on the Access Array™ system. Since its launch in 2009, Access Array has been an indispensible support tool for high-throughput sequencing, targeted enrichment and library preparation for some of the world’s leading laboratories.
Browse our catalog of related publications to learn how Access Array enables researchers around the globe to do more with much less when it comes to library prep.
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FAQs
We’ve answered some of the most relevant recurring questions about Access Array to ensure that you have the information you need to succeed in your research. For additional information, check out the tutorials and technical documents or inquire about one of our training programs.
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Applications
- What materials are included in each kit?
- What information is available about the performance of the Advanta CFTR NGS Library Prep Assay?
- Can you provide a summary of the assay workflow?
- What data analysis and bioinformatic pipeline tools are available?
- What is the cost of the product and what is the effective cost per sample?
- How do I order the product?
- Can I evaluate the product prior to making a purchase commitment?
- How does this assay compare to competitor assays with regard to performance, cost and workflow, and what sets Fluidigm apart from the competition?
- Which makes and models of NGS systems are supported by the assay?
- What other assays are available for the Juno system and Access Array system?
- What equipment is required to use the product?
- How many samples can be processed per workday, and what is the total turnaround time from start to finish?
- How do I estimate read depth coverage?
- How many samples can be processed in a single batch?
- What are the sample requirements?
- Does your product include sample barcodes?
- Which CFTR variants does the Advanta CFTR NGS Library Prep Assay cover and how were they chosen?
- How many amplicons are generated by the Advanta CFTR NGS Library Prep Assay, and what is the size?
- Have you tested any materials that could be used as controls, and can you recommend a source for control materials?
- Can Fluidigm sequester specific lots of reagents and/or consumables for my lab, and if so, what is the process for sequestering lots?
- What materials are needed to use the kit but are not included in the kit?
- How many unique sample barcodes are available?
- Do you have any publications from users of the product?
- How did you handle the detection of the large exonic deletions within your design?
- Can I include sample identification markers such as the Fluidigm SNP Trace sample ID panel?
- What species and targets do you design assays to?
- Can I automate use of the product with my liquid handler?
- What level of manual input is required?
- What level of skill is required to perform the assay and how complex is the workflow?
- When is the use of preamplification recommended?
- Can I add other variants of interest that are not covered by the Advanta CFTR NGS Library Prep Assay?
- Your CF design only uses a portion of the assay inlets within the IFC. What am I supposed to do with the unused assay inlets? View more
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Software
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Design
- What materials are included in each kit?
- What information is available about the performance of the Advanta CFTR NGS Library Prep Assay?
- How do I estimate read depth coverage?
- How many samples can be processed in a single batch?
- What are the sample requirements?
- Does your product include sample barcodes?
- Which CFTR variants does the Advanta CFTR NGS Library Prep Assay cover and how were they chosen?
- How many amplicons are generated by the Advanta CFTR NGS Library Prep Assay, and what is the size?
- Have you tested any materials that could be used as controls, and can you recommend a source for control materials?
- What materials are needed to use the kit but are not included in the kit?
- How many unique sample barcodes are available?
- How did you handle the detection of the large exonic deletions within your design?
- Can I include sample identification markers such as the Fluidigm SNP Trace sample ID panel?
- What species and targets do you design assays to?
- What if I want singleplex assays?
- When is the use of preamplification recommended?
- Can I add other variants of interest that are not covered by the Advanta CFTR NGS Library Prep Assay?
- Your CF design only uses a portion of the assay inlets within the IFC. What am I supposed to do with the unused assay inlets?
- What is the process for redesigning assays?
- What are the maximum supported amplicon count and amplicon size?
- How do I determine the status of D3 assay design requests?
- What species and targets do you design Delta Gene gene expression assays to?
- Do you have any advice on primer design?
- Does Fluidigm provide a sequencing service? View more
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Orders
- What is the cost of the product and what is the effective cost per sample?
- How do I order the product?
- How do I place orders?
- How do I place an order online?
- How can I see prices?
- What is available through online ordering?
- How do I set up an online ordering account?
- What payment options do you accept?
- What are the delivery options?
- Can Fluidigm sequester specific lots of reagents and/or consumables for my lab, and if so, what is the process for sequestering lots?
- What is included in the IFC Controller Premium Certification Service Plan?
- Can Fluidigm design assays targeting introns?
- What is the design turnaround time?
- What is provided with my order of Access Array Target-Specific Primers?
- How do I find my local distributor?
- How do I cancel, add or modify orders?
- How do I sign in to my account?
- How do I arrange credit terms?
- What currencies do you accept?
- How do I request a quote?
- What is the process for redesigning assays?
- How do I determine the status of D3 assay design requests?
- What information is provided in the Customer Informatics Packet?
- What is included in the IFC Controller and FC1 Cycler Depot Repair Service Plans?
- What are the key benefits of the IFC Controller and FC1 Cycler Depot Repair Service Plans?
- How do I supply my sales tax exemption?
- Are splice sites included in the exon design?
- Do you design assays for repetitive regions?
- What defines difficult genomic regions, and can primers be designed to cover these regions?
- What species are available for custom assay design?
- How do I disable my account?
- Can I use any email address for online ordering?
- How can I request a copy of my invoice?
- How can I track shipments?
- How do I view my online order history?
- Can I slightly increase the size of amplicon X to explore the end of intron Y near exon Z?
- What information is provided in the Customer Informatics Packet for the Access Array Target-Specific Primers?
- Does Fluidigm provide a sequencing service?
- I have a quote from my sales representative. Can I use that quote online?
- Can I order for multiple labs? View more
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Technical Service
- What is included in the IFC Controller Premium Certification Service Plan?
- What are the key benefits of the IFC Controller Premium Certification Service Plan?
- What is included in the IFC Controller and FC1 Cycler Depot Repair Service Plans?
- What are the key benefits of the IFC Controller and FC1 Cycler Depot Repair Service Plans?
- How do I subscribe to the Fluidigm newsletter?
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Consumables
- What is the shelf life for IFCs and Control Line Fluid?
- Can Fluidigm design assays targeting introns?
- What is the design turnaround time?
- What is provided with my order of Access Array Target-Specific Primers?
- Can I use the LP 192.24 IFC on the AX Controller?
- Can I use my existing Access Array kit and assays on Juno with the Juno LP 192.24 IFC?
- Can the IFCs be centrifuged to get rid of bubbles?
- If I have multiple IFC runs, can I prepare my sample mix and assay mix the day before? A week before?
- How much time can elapse between loading an IFC and thermal cycling?
- Can I reuse an IFC? Can I use part of an IFC, and then use the other part in another experiment?
- How quickly do I need to use the IFC after the foil pouch is opened?
- Why can’t I load the samples in the assay inlets and the assays in the sample inlets?
- How should I store my genotyping assays?
- Are splice sites included in the exon design?
- Do you design assays for repetitive regions?
- What defines difficult genomic regions, and can primers be designed to cover these regions?
- What species are available for custom assay design?
- What factors does D3 assay design consider when designing primers for Access Array?
- What equipment is required for Access Array target-specific amplification?
- How many 48.48 Access Array IFCs can I run with the provided assays?
- What are Access Array Target-Specific Primers?
- What are the performance specifications for Access Array Target-Specific Primers?
- Can I slightly increase the size of amplicon X to explore the end of intron Y near exon Z?
- What information is provided in the Customer Informatics Packet for the Access Array Target-Specific Primers? View more
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training
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Workflows
- What level of coverage can I expect on each sequencing platform?
- Can you provide a summary of the assay workflow?
- How many samples can be processed per workday, and what is the total turnaround time from start to finish?
- What is the maximum length for Access Array amplicons?
- How much volume is retrieved for each sample on an Access Array?
- How does multiplexing work on the Access Array?
- Can I sequence Access Array products on the Ion Torrent sequencer? Do you have a protocol?
- How long are the CS1 and CS2 universal tags, barcodes and adapters for 454 and Illumina sequencers?
- Can I use the LP 192.24 IFC on the AX Controller?
- What level of manual input is required?
- What level of skill is required to perform the assay and how complex is the workflow?
- If I have multiple IFC runs, can I prepare my sample mix and assay mix the day before? A week before?
- How much time can elapse between loading an IFC and thermal cycling?
- What if I don’t have exactly 48 or 96 samples or assays? Can I load water into my empty inlets?
- How should I store my genotyping assays?
- I have started my Access Array run but cannot stay until the end. Where can I stop the experiment to finish later?
- How much of my FFPE sample should I load on the Access Array?
- Can I run a gel on my harvested Access Array products or systems other than Agilent Bioanalyzer 2100? View more
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Chemistry
- What is the maximum length for Access Array amplicons?
- How much volume is retrieved for each sample on an Access Array?
- How does multiplexing work on the Access Array?
- Can I sequence Access Array products on the Ion Torrent sequencer? Do you have a protocol?
- How long are the CS1 and CS2 universal tags, barcodes and adapters for 454 and Illumina sequencers?
- Can Fluidigm design assays targeting introns?
- How should I store my Advanta NGS Library Prep Assay primers?
- What if I want singleplex assays?
- Can I use my existing Access Array kit and assays on Juno with the Juno LP 192.24 IFC?
- What are the maximum supported amplicon count and amplicon size?
- I have started my Access Array run but cannot stay until the end. Where can I stop the experiment to finish later?
- Are splice sites included in the exon design?
- Do you design assays for repetitive regions?
- What defines difficult genomic regions, and can primers be designed to cover these regions?
- What species are available for custom assay design?
- What factors does D3 assay design consider when designing primers for Access Array?
- How much of my FFPE sample should I load on the Access Array?
- Can I slightly increase the size of amplicon X to explore the end of intron Y near exon Z? View more
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Documents
We endeavor to think of everything when we develop a system, so we hope you’ll find all the details and explanations you need in the technical and support documents below. Simply click a title to open the document in your browser.
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Protocols
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Publications
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Quick References
- Access Array 48.48 IFC 4-Primer Amplicon Tagging Workflow
- Control Line Fluid Loading Procedure
- FC1 Cycler Setup
- Access Array 48.48 IFC 2-Primer Workflow Quick Reference
- Access Array Multiplex 20X Primer Solution Preparation
- Advanta CFTR NGS Library Preparation on the LP 48.48 IFC with Access Array Quick Reference
- Advanta NGS Library Preparation on the LP 48.48 IFC with Access Array Quick Reference
- Access Array—Generate Tagged Primers Workbook View more
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Release Notes
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Safety Data Sheets
- Advanta™ CFTR NGS Library Prep Assay—LP 48.48, 2 IFCs
- Fluidigm Custom Target Specific Assays Safety Data Sheet
- Control Line Fluid Safety Data Sheet
- Advanta™ NGS Preamp Reagent Kit
- Advanta™ IO Gene Expression Reagent Kit—GE 24.192
- Access Array™ Barcode Library for Illumina Sequencers - 384 (Bidirectional) Safety Data Sheet
- Access Array™ Barcode Library for Illumina Sequencers - 384 (Single Direction) Safety Data Sheet
- Access Array™ Barcode Library for Ion Torrent PGM™ Sequencer - 96 Safety Data Sheet
- 20X Access Array™ Loading Reagent Safety Data Sheet
- AA 192.24 Sample Loading Reagent Safety Data Sheet
- Hydration Reagent v2 Safety Data Sheet
- 1X Access Array™ Harvest Solution Safety Data Sheet
- Targeted DNA Seq Library Reagent Kit v2 – 192.24 Safety Data Sheet View more
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Technical Notes
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User Guides
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Product Inserts
- Advanta NGS Library Prep Reagent Kit—LP 48.48, 2 IFCs Product Insert
- Advanta CFTR NGS Assay Pools Product Insert
- Advanta CFTR NGS Preamp Pool Product Insert
- Advanta NGS Library Prep Reagent Bundle—LP 48.48, 10 IFCs Product Insert
- Targeted DNA Seq Library Adapter Set Product Insert
- LP—48.48 Control Line Fluid Product Insert
- LP—48.48 IFC with Accessories Product Insert
- Advanta NGS Preamp Reagent Kit—96 Rxn Product Insert
- LP—48.48 Barrier Tape
- Access Array Target-Specific Primers View more
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Training & Services
At Fluidigm, we’re proud to create biotech tools that enable our customers to seek truth in life sciences. We’re dedicated to working with you to empower you however we can. That’s why we’ve developed a range of training programs to help you make the most of your Access Array system.
Fluidigm is dedicated to providing industry-leading technology and tools that enable you to achieve more in life sciences. And to help you stay ahead, we stand behind our work, offering exceptional service plans and options to keep your Access Array instrument in top condition.
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Consumables
Achieve high-quality results with fewer errors and more sequence-ready PCR product using innovative IFCs and assays designed especially for Access Array.
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Contact Support
CyTOF & Helios
T: 650 266 6100
Toll Free: 866 358 4354 in the US
Press 5 for CyTOF inquiries when operator is logged offReach out to our global support team here.