This kit enables the preparation of NGS-ready libraries from 27 exons and select intronic regions of the cystic fibrosis transmembrane conductance regulator gene (CFTR) for subsequent sequencing analysis. The kit includes sufficient targeted DNA sequencing library reagents, CFTR NGS Assay Pools, IFCs, and Control Line Fluid to generate 96 libraries.
Each kit contains:
- 1 tube of TSP Sample Loading Reagent v2, 200 µL
- 1 tube of TSP Adapter Mix, 120 µL
- 1 tube of PCR Water, 1.8 mL
- 1 bottle of DNA Dilution Reagent, 3.0 mL
- 1 bottle of TSP Harvest Reagent, 10 mL
- 1 tube of TSP Assay Loading Reagent, 75 µL
- 1 tube of 4X TSP Master Mix, 210 µL
- 1 tube of TSP DNA Polymerase, 50 µL
- 1 set of Advanta™ CFTR NGS Assay Pools, 8 tubes per set, 10 µL/tube
- 2 IFCs, LP 48.48
- 4 syringes of LP Control Line Fluid (300 µL/syringe)
Product insert available.