Consumables

Advanta™ RNA-Seq NGS Library Prep Kit

This kit uses a poly(A) enrichment approach to prepare NGS-ready, dual-indexed, full-length cDNA libraries from total RNA. The kit contains sufficient 48.Atlas™ IFCs, reagents, sample indexes, and Control Line Fluid to process 4 IFCs (up to 192 samples per kit).


Kit Contains:

4 IFCs, 48.Atlas

8 syringes of Control Line Fluid (150 μL/syringe)

Module 1 (–80 °C)

  • 1 tube of TSO Mix, 50 μL
Module 2 (–20 °C)
  • 1 tube of DNA Polymerase, 130 μL
  • 2 bottles of Harvest Reagent Type 1, 7.6 mL
  • 1 tube of Loading Reagent Type 1, 200 μL
  • 1 tube of Enhancer Buffer, 100 μL
  • 1 tube of mRNA Binding Buffer, 1 mL
  • 1 tube of N6, 15 μL
  • 1 bottle of PCR Buffer, 3.5 mL
  • 1 tube of PCR2 Primer, 65 μL
  • 1 tube of Post-Capture Buffer, 1 mL
  • 1 tube of Pre-Capture Buffer, 120 μL
  • 1 tube of RNase Inhibitor, 20 μL
  • 1 tube of RT Buffer, 50 μL
  • 1 tube of RT Enzyme, 24 μL
  • 1 tube of Tris Buffer, 1.25 mL
Module 3 (–20 °C)
  • 1 tube of 3′ PCR Primer HT Index 1, 15 µL
  • 1 tube of 3′ PCR Primer HT Index 2, 15 µL
  • 1 tube of 3′ PCR Primer HT Index 3, 15 µL
  • 1 tube of 3′ PCR Primer HT Index 4, 15 µL
  • 1 tube of 3′ PCR Primer HT Index 5, 15 µL
  • 1 tube of 3′ PCR Primer HT Index 1, 15 µL
  • 1 tube of 3′ PCR Primer HT Index 6, 15 µL
  • 1 tube of 3′ PCR Primer HT Index 7, 15 µL
  • 1 tube of 3′ PCR Primer HT Index 8, 15 µL

  • 1 tube of 5′ PCR Primer HT Index 1, 15 µL
  • 1 tube of 5′ PCR Primer HT Index 2, 15 µL
  • 1 tube of 5′ PCR Primer HT Index 3, 15 µL
  • 1 tube of 5′ PCR Primer HT Index 4, 15 µL
  • 1 tube of 5′ PCR Primer HT Index 5, 15 µL
  • 1 tube of 5′ PCR Primer HT Index 6, 15 µL
  • 1 tube of 5′ PCR Primer HT Index 7, 15 µL
  • 1 tube of 5′ PCR Primer HT Index 8, 15 µL
  • 1 tube of 5′ PCR Primer HT Index 9, 15 µL
  • 1 tube of 5′ PCR Primer HT Index 10, 15 µL
  • 1 tube of 5′ PCR Primer HT Index 11, 15 µL
  • 1 tube of 5′ PCR Primer HT Index 12, 15 µL
  • 1 bottle of Nuclease-Free Water, 5 mL
Module 4 (4 °C)
  • 1 tube of RNA-Seq Beads, 150 μL


Product inserts available.


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