Product

Consumables

Maxpar® Human Helper T Cell Phenotyping Panel Kit, 15 Marker—25 Tests

The Maxpar Human Helper T Cell Phenotyping Panel Kit is for the identification and phenotyping of human CD4+ helper T cell subsets, including T helper 1 (Th1), Th2, Th17, Th22, T follicular helper (TFH) and T regulatory (Treg). Differentiation of CD4+ T cells into functionally distinct helper T subsets is essential for normal immunoregulation. These subsets are specified by extrinsic and intrinsic cues, and the resultant cell populations acquire stable phenotypes defined by expression of signature cytokines, master regulator transcription factors, and characteristic cell surface phenotypes. Originally, CD4+ T cells were viewed as having only 2 major fates: T helper 1 (Th1) cells, which express T-bet and selectively produce interferon IFNγ, and Th2 cells, which express GATA3 and produce interleukin IL-4. Although this represented a simple model system for understanding basic principals in cellular immunology, the Th1/Th2 paradigm failed to explain several facets of immunity and autoimmunity. The recognition of cells that selectively produce IL-17 and the transcription factor RORγt (Th17 cells) led to renewed interest in the topic of helper T cell differentiation. Treg cells are another CD4+ lineage with essential immunosuppressive functions that express the master transcription factor Foxp3. Newer fates for helper T cells, including Th22 cells, continue to be identified, with nomenclature based on production of their signature cytokines. The newest lineage of CD4 T cells, follicular helper T (TFH) cells, are crucial for providing B cell help by promoting class-switching of B cells. They are defined by expression of master regulator Bcl-6 and effector cytokine IL-21, along with key surface molecules (PD-1, CXCR5, and ICOS).

Target

Clone

Metal

CCR6 G034E3 141Pr
CCR5 NP-6G4 144Nd
CD8 RPA-T8 146Nd
ICOS C398.4A 151Eu
CD45RA HI100 153Eu
CD3 UCHT1 154Sm
CXCR3 G025H7 156Gd
CCR4 L291H4 158Gd
CD161 HP-3G10 159Tb
CD45RO UCHL1 165Ho
CD25 2A3 169Tm
CXCR5 RF8B2 171Yb
CD4 SK3 174Yb
PD-1 EH12.2H7 175Lu
CD127 A019D5 176Yb
  • Species:
    Human
  • Markers:
    15

 

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