Sample Preparation

Enable your translational and clinical research with quality nucleic acids

Product

While formalin-fixed, paraffin-embedded (FFPE) tissue samples are a valuable source for biomarker investigation, DNA and RNA extraction from FFPE samples has been challenging due severe degradation of nucleic acids during the fixation process and limited size of discrete samples. To obtain meaningful data during gene expression analysis and next generation sequencing (NGS), it is critical to use purification methods that overcome these limitations of FFPE samples. Also, qualification of RNA or DNA enables optimization of nucleic acid input for downstream applicating and is critical for a successful NGS library construction.

Improve your RNA quality for gene expression application with Fluidigm FFPE RNA extraction kit.

What's New

New Biomark assay enables efficient interrogation of the tumor microenvironment

Tumor gene expression has proven effective in measuring immune response during cancer progression and therapeutic response. As emerging therapies reveal new biomarkers and expand the need for samples, the costs and labor required to complete this important work also rise. Cancer researchers now have a reliable, sensitive and cost-effective tool for identifying gene expression signatures from immune and cancer cells: the new Advanta™ IO Gene Expression Assay, used with the Biomark™ HD system

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Improving The Process

Processing FFPE samples can be challenging due to limited sample quantities or fragmented RNA transcripts caused by nucleic acid degradation as a result of tissue fixation and storage methods. In addition, most extraction kits rely on column purification to remove RNA and DNA, causing significant nucleic acid loss during the isolation process. To address this issue, the Advanta FFPE RNA Extraction Kit uses a column-free approach to effectively recover quality RNA from FFPE samples.

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Methods

RNA Extraction

Obtain meaningful data in your gene expressing assay using high-molecular weight RNA isolated using a xylene-free bead-based method.

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